EARLY FLOWERING IN SHORT DAYS (EFS) regulates the seed size in Arabidopsis
EARLY FLOWERING IN SHORT DAYS (EFS) regulates the seed size in Arabidopsis
复制标题
短日提早开花 (EFS) 调节拟南芥种子大小
DOI：
10.1007/s11427-017-9236-x
复制
发表时间：
2018-02-01
影响因子：
9.1
通讯作者：
Sun，Qianwen
中科院分区：
文献类型：
--
作者：
Cheng，Lingling;Shafiq，Sarfraz;Sun，Qianwen
转录后修饰，包括组蛋白修饰和DNA甲基化，改变染色质景观来调节基因表达，从而控制植物的各种细胞过程。短日照早开花（EFS）是拟南芥中H3 K36甲基化的主要贡献者，对植物发育具有重要意义。在这里，我们发现EFS在胚胎形态发生的不同阶段表达，并且EFS突变体产生更大的胚胎，从而导致扩大的种子。进一步的分析表明，印迹基因MOP9.5在启动子区被低甲基化，其表达在efs突变体中被去抑制。MOP9.5启动子的表观遗传修饰是其重要特征之一，我们发现在efs突变体中，随着H3 K36 me 3的增加，H3 K27 me 3和H3 K9 me 2的水平降低。该数据表明H3 K36 me 3与DNA甲基化和/或H3 K27 me 3之间在MOP9.5下的拮抗调节。我们的研究结果进一步表明，母本和父本的EFS等位基因负责种子大小的调节，揭示了一个新的功能，EFS在植物发育。
Post-transcriptional modifications, including histone modifications and DNA methylation, alter the chromatin landscape to regulate gene expression, thus control various cellular processes in plants. EARLY FLOWERING IN SHORT DAYS (EFS) is the major contributor for H3K36 methylation in Arabidopsis and is important for plant development. Here, we find that EFS is expressed in different stages of embryo morphogenesis, and the efs mutant produces larger embryo that results in enlarged seeds. Further analysis reveals that an imprinted gene MOP9.5 is hypomethylated at the promoter region and its expression is derepressed in efs mutant. MOP9.5 promoter is marked by various epigenetic modifications, and we find that following the increase of H3K36me3, H3K27me3 and H3K9me2 levels are reduced in efs mutant. This data indicates an antagonistic regulation between H3K36me3 and DNA methylation, and/or H3K27me3 at MOP9.5. Our results further show that both maternal and paternal EFS alleles are responsible for the seed size regulation, which unraveled a novel function of EFS in plant development.