Retinal Oximetry Using Intravitreal Illumination
Retinal Oximetry Using Intravitreal Illumination
复制标题
使用玻璃体内照明进行视网膜血氧测定
DOI：
10.1080/02713680600760493
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发表时间：
2006
影响因子：
2
通讯作者：
R. Park
中科院分区：
文献类型：
--
作者：
D. Salyer;N. Beaudry;S. Basavanthappa;K. Twietmeyer;M. Eskandari;K. Denninghoff;R. Chipman;R. Park
目的：证明使用玻璃体内照明对猪的动脉和静脉进行光谱视网膜血氧测量。在玻璃体切除术后，测量视网膜动脉和静脉饱和度，以获得一定范围的吸入O2水平。方法：对两只雌性美国约克郡家猪进行睫状体平坦部玻璃体切除术和玻璃体内操作。将来自扫描单色仪的光耦合到插入玻璃体中的光纤眼内照明器中。倾斜照明视网膜血管，最大限度地减少血管闪烁。当猪的动脉血氧饱和度以大约10%的递减从100%降低到67%时，获得视网膜脉管系统的多光谱图像。视网膜血管光谱用于计算选定动脉和静脉中的氧饱和度。动脉氧饱和度使用从放置在股动脉中的Swan-Ganz导管抽取的血液的血气分析来校准。结果如下：使用玻璃体内光纤照明器的视网膜血管的倾斜照明提供了通常在眼底图像中看到的中央血管闪烁的大幅减少，从而简化了光谱数据的分析。血管阴影从血管图像中移位，简化了眼睛中的光路。使用简化的光路简化的全光谱分析，我们计算视网膜血管饱和度。闪烁的减少允许增加测量视网膜血管光谱光密度的准确性。在玻璃体切除术后不久观察到异常低的视网膜静脉血氧饱和度。结论：使用玻璃体内照明的视网膜血氧饱和度测定已经得到证实。作为一种研究工具，玻璃体内照明解决了使用经角膜照明进行视网膜血氧测定时遇到的几个困难。
Purpose: To demonstrate spectroscopic retinal oximetry measurements on arteries and veins in swine using intravitreal illumination. Retinal arterial and venous saturations are measured for a range of inspired O2 levels after pars plana vitrectomy. Methods: Pars plana vitrectomy and intravitreal manipulations were performed on two female American Yorkshire domestic swine. Light from a scanning monochromator was coupled into a fiberoptic intraocular illuminator inserted into the vitreous. The retinal vessels were illuminated obliquely, minimizing vessel glints. Multispectral images of the retinal vasculature were obtained as the swine's arterial blood oxygen saturation was decreased from 100% to 67% in decrements of approximately 10%. Retinal vessel spectra were used to calculate oxygen saturation in selected arteries and veins. Arterial oxygen saturations were calibrated using blood gas analysis on blood drawn from a Swan-Ganz catheter placed in the femoral artery. Results: Oblique illumination of retinal vessels using an intravitreal fiberoptic illuminator provided a substantial reduction in the central vessel glint usually seen in fundus images, thus simplifying the analysis of spectral data. The vessel shadows were displaced from the vessel image simplifying the light paths in the eye. Using a full spectral analysis simplified by the light path reductions, we calculated retinal vessel saturations. The reduction of glint allowed for increased accuracy in measuring retinal vessel spectral optical density. Abnormally low retinal venous oxygen saturations were observed shortly after pars plana vitrectomy. Conclusions: Retinal oximetry using intravitreal illumination has been demonstrated. As a research tool, intravitreal illumination addresses several difficulties encountered when performing retinal oximetry with transcorneal illumination.