Surrogate analyte-based quantification of main endocannabinoids in whole blood using liquid chromatography-tandem mass spectrometry
Surrogate analyte-based quantification of main endocannabinoids in whole blood using liquid chromatography-tandem mass spectrometry
复制标题
使用液相色谱-串联质谱法对全血中主要内源性大麻素进行基于替代分析物的定量
DOI：
10.1002/bmc.4439
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发表时间：
2019-03-01
影响因子：
1.8
通讯作者：
Shen, Min
中科院分区：
文献类型：
--
作者：
Dong, Xiaoru;Li, Liliang;Shen, Min
内源性大麻素 (eCB) 是内源性大麻素系统的内源性配体，已知可调节多种生理和行为过程。先前的研究已经开发了检测主要 eCB 的方法，包括花生四烯乙醇酰胺 (AEA) 和 2-花生四烯酰甘油 (2-AG)，主要存在于血清或血浆中。由于缩短了分离程序并降低了制备过程中 2-AG 异构化的风险，全血是用于 eCB 分析的优质生物材料。在本研究中，开发了一种基于替代分析物的液相色谱-串联质谱测定法，用于使用最多 100 μL 全血测量 AEA、2-AG 及其异构体 1-花生四烯酰甘油 (1-AG)。使用反相柱和梯度洗脱实现色谱分离。使用电喷雾电离源以选定的反应监测模式进行检测。三种 eCB 的检测限为 0.05-0.1 ng/mL。在浓度范围内观察到良好的线性。测定内和测定间准确度和精密度
Endocannabinoids (eCBs) are endogenous ligands of the endocannabinoid system that are known to regulate several physiological and behavioral processes. Previous studies have developed methods for the detection of main eCBs including arachidonylethanolamide (AEA) and 2-arachidonoylglycerol (2-AG), mostly in serum or plasma. Whole blood is a superior biomaterial for eCBs analysis owing to the nature of the shortened isolation procedure and decreased risk of 2-AG isomerization during preparation. In this study, a surrogate analyte-based liquid chromatography-tandem mass spectrometry assay was developed for the measurement of AEA, 2-AG and its isomer 1-arachidonoylglycerol (1-AG) using a maximum of 100 mu L whole blood. Chromatographic separation was achieved using a reverse-phase column and a gradient elution. Detection was performed in selected reaction monitoring mode with an electrospray ionization source. The limits of detection of three eCBs were 0.05-0.1 ng/mL. Good linearity was observed over the concentration range. Intra- and inter-assay accuracy and precision were