Suppressors of transforming growth factor-beta pathway mutants in the Caenorhabditis elegans dauer formation pathway.
Suppressors of transforming growth factor-beta pathway mutants in the Caenorhabditis elegans dauer formation pathway.
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发表时间：
2000-11
期刊：
影响因子：
3.3
通讯作者：
Takao Inoué;James H. Thomas
中科院分区：
文献类型：
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作者：
Takao Inoué;James H. Thomas
dauer 是一种发育停滞的秀丽隐杆线虫第三幼虫阶段。进入这种状态受到环境因素的调节，包括温度、食物和组成性分泌的多尔信息素的浓度。从遗传学上来说，已经发现了调节这一过程的三个平行途径。其中，第 2 组通路包括基因 daf-1、daf-3、daf-4、daf-5、daf-7、daf-8 和 daf-14，通过 ASI 化学感应神经元介导环境信号的转导，并编码 TGF-β 相关信号通路。为了鉴定在该途径中起作用的其他基因，我们对 daf-1、daf-8 和 daf-14 中的突变抑制子进行了筛选。从总共 36 个突变中，鉴定出 7 个互补组。三个互补组对应于先前描述的基因 daf-3、daf-5 和 daf-12。三个对应于新基因scd-1、scd-2和scd-3。本文介绍了这些 scd 基因的遗传分析。第四个互补组以 sa315 单突变为代表，它影响 daf-2/age-1 胰岛素相关信号通路。
The dauer is a developmentally arrested alternative third larval stage of Caenorhabditis elegans. Entry into this state is regulated by environmental cues, including temperature, food, and the concentration of constitutively secreted dauer pheromone. Genetically, three parallel pathways have been found that regulate this process. Of these, the group 2 pathway, which includes the genes daf-1, daf-3, daf-4, daf-5, daf-7, daf-8, and daf-14, mediates the transduction of environmental signals through the ASI chemosensory neuron and encodes a TGF-beta-related signaling pathway. To identify additional genes that function in this pathway, we carried out a screen for suppressors of mutations in daf-1, daf-8, and daf-14. From the total of 36 mutations, seven complementation groups were identified. Three complementation groups correspond to the previously described genes daf-3, daf-5, and daf-12. Three correspond to novel genes scd-1, scd-2, and scd-3. Genetic analysis of these scd genes is presented here. A fourth complementation group was represented by a single mutation sa315, which affects the daf-2/age-1 insulin-related signaling pathway.