EPIDERMAL GROWTH-FACTOR ATTENUATES JEJUNAL MUCOSAL INJURY-INDUCED BY OLEIC-ACID - ROLE OF MUCUS
EPIDERMAL GROWTH-FACTOR ATTENUATES JEJUNAL MUCOSAL INJURY-INDUCED BY OLEIC-ACID - ROLE OF MUCUS
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DOI：
10.1152/ajpgi.1994.267.6.g1067
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发表时间：
1994-12-01
影响因子：
4.5
通讯作者：
KVIETYS, PR
中科院分区：
文献类型：
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作者：
ISHIKAWA, S;CEPINSKAS, G;KVIETYS, PR
表皮生长因子（EGF）存在于胆道、胰腺和布鲁纳腺分泌物中。本研究的目的是评估EGF对脂质诱导的粘膜损伤的影响。麻醉大鼠空肠近端插管，用乳化油酸（40 mM油酸配20 mM牛磺胆酸钠，pH 6.0）灌注管腔。通过测量cr -51标记的EDTA的血-腔清除率来监测粘膜上皮的完整性。腹腔灌注乳化脂质增加EDTA清除率。在脂质乳中加入EGF （0.5 ng/ml）可以改善脂质诱导的EDTA清除率的增加。单独灌注EGF可刺激杯状细胞分泌粘液。这种作用被阿托品所消除。此外，在阿托品化的动物中，存在1)脂质诱导损伤的夸大和2)EGF保护作用的丧失。我们的研究结果提供了证据，支持EGF通过刺激粘液产生，在一定程度上防止脂质诱导的粘膜损伤的假设。
Epidermal growth factor (EGF) is present in biliary, pancreatic, and Brunner's gland secretions. The aim of the present study was to assess the effects of EGF on lipid-induced mucosal injury. The proximal jejunum of anesthetized rats was cannulated for perfusion of the lumen with emulsified oleic acid (40 mM oleic acid in 20 mM sodium taurocholate; pH 6.0). Mucosal epithelial integrity was monitored by measuring the blood-to-lumen clearance of Cr-51-labeled EDTA. Perfusion of the lumen with emulsified lipid increased EDTA clearance. Addition of EGF (0.5 ng/ml) to the lipid emulsion ameliorated the lipid-induced increase in EDTA clearance. Perfusion of the lumen with EGF alone stimulated mucus secretion from goblet cells. This effect of EGF was abolished by atropine. In addition, in atropinized animals there was 1) an exaggeration of the lipid-induced injury and 2) a loss of the protective effect of EGF. Our findings provide evidence supporting the hypothesis that EGF provides protection against lipid-induced mucosal injury, in part, by stimulating mucus production.