Glutathione-independent prostaglandin D2 synthase in ram and stallion epididymal fluids: origin and regulation.
Glutathione-independent prostaglandin D2 synthase in ram and stallion epididymal fluids: origin and regulation.
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DOI：
10.1095/biolreprod60.3.558
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发表时间：
1999-03
影响因子：
3.6
通讯作者：
S. Fouchécourt;F. Dacheux;J. Dacheux
中科院分区：
文献类型：
--
作者：
S. Fouchécourt;F. Dacheux;J. Dacheux
双向电泳后的显微测序显示，公羊和种马的附睾液中存在一种主要的蛋白质--谷胱甘肽非依赖性前列腺素D2合成酶(PGDS)。在这个附睾区，PGDS是一种多态化合物，其分子质量约为30 kDa，等电点为4~7。在公羊和种马中，PGDS分别占该区域存在的管腔蛋白总量的15%和8%。通过体外生物合成判断蛋白质的分泌，通过Northern印迹分析研究其mRNA的存在，仅限于附睾头的近端。使用一种针对合成肽的特异性多克隆抗体，可以在附睾部发现PGDS，其浓度向尾部递减。Western blotting还检测到睾丸液和精浆中的PGDS。去势和结扎传出管导致公羊PGDS基因表达和分泌减少。去势后1mo未检测到PGDS基因的表达，补充睾酮可使其恢复正常。这项研究表明，PGDS存在于整个男性生殖道的精子环境中。它在精子成熟和/或保护中的作用尚不清楚。
Microsequencing after two-dimensional electrophoresis revealed a major protein, glutathione-independent prostaglandin D2 synthase (PGDS) in the anterior epididymal region fluid of the ram and stallion. In this epididymal region, PGDS was a polymorphic compound with a molecular mass around 30 kDa and a range of pI from 4 to 7. PGDS represented 15% and 8% of the total luminal proteins present in this region in the ram and stallion, respectively. The secretion of the protein as judged by in vitro biosynthesis, and the presence of its mRNA as studied by Northern blot analysis, were limited to the proximal caput epididymidis. Using a specific polyclonal antibody raised against a synthetic peptide, PGDS was found throughout the epididymis, decreasing in concentration toward the cauda region. PGDS was also detected in the testicular fluid and seminal plasma by Western blotting. Castration and efferent duct ligation in the ram led to a decrease in PGDS mRNA and secretion. PGDS mRNA was not detected in the stallion 1 mo after castration, and it was restored by testosterone supplementation. This study showed that PGDS is present in the environment of spermatozoa throughout the male genital tract. Its function in the maturation and/or protection of spermatozoa is unknown.