Spatial Regionalization and Heterochrony in the Formation of Adult Pallial Neural Stem Cells
Spatial Regionalization and Heterochrony in the Formation of Adult Pallial Neural Stem Cells
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DOI：
10.1016/j.devcel.2014.05.012
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发表时间：
2014-07-28
影响因子：
11.8
通讯作者：
Foucher, Isabelle
中科院分区：
文献类型：
--
作者：
Dirian, Lara;Galant, Sonya;Foucher, Isabelle
对于成体神经干细胞（aNSC）的胚胎起源和相关异质性知之甚少。我们使用条件遗传追踪，通过细胞类型特异性启动子或聚焦激光解笼以全局或镶嵌方式激活，结合基因表达分析和Notch失效，来解决斑马鱼成年端脑中的这个问题。我们报告说，成人大脑皮层的生发区起源于胚胎祖细胞的两种不同亚型，并整合了两种 aNSC 形成模式。背内侧 aNSC 源自胚胎端脑活跃的神经源性放射状胶质细胞的扩增。相反，外侧aNSC群是通过在大脑皮层边缘从后端脑顶的离散神经上皮祖细胞池逐步添加而形成的，在胚胎后阶段被激活并持续终生。大脑皮层生发区的这种双重起源使得大脑皮层区域能够在时间上组织起来，作为并置隔室的拼凑而成。
Little is known on the embryonic origin and related heterogeneity of adult neural stem cells (aNSCs). We use conditional genetic tracing, activated in a global or mosaic fashion by cell type-specific promoters or focal laser uncaging, coupled with gene expression analyses and Notch invalidations, to address this issue in the zebrafish adult telencephalon. We report that the germinal zone of the adult pallium originates from two distinct subtypes of embryonic progenitors and integrates two modes of aNSC formation. Dorsomedial aNSCs derive from the amplification of actively neurogenic radial glia of the embryonic telencephalon. On the contrary, the lateral aNSC population is formed by stepwise addition at the pallial edge from a discrete neuroepithelial progenitor pool of the posterior telencephalic roof, activated at postembryonic stages and persisting lifelong. This dual origin of the pallial germinal zone allows the temporally organized building of pallial territories as a patchwork of juxtaposed compartments.