Influences of vascularization and osteogenic cells on heterotopic bone formation within a madreporic ceramic in rats
Influences of vascularization and osteogenic cells on heterotopic bone formation within a madreporic ceramic in rats
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DOI：
10.1097/01.prs.0000055044.14093.ea
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发表时间：
2003-05-01
影响因子：
3.6
通讯作者：
Vilamitjana-Amedee, J
中科院分区：
文献类型：
--
作者：
Pelissier, P;Villars, F;Vilamitjana-Amedee, J
骨重建生物材料的研究已经导致了将多孔陶瓷与骨髓基质细胞结合在一起的复杂的成骨复合材料。本研究的目的是评估这种复合材料直接血管化对成骨的影响，以及在异位肌肉部位产生血管化骨替代移植的能力。将64种珊瑚生物材料植入32只Fisher大鼠体内，分为四种情况：(1)单独（参照组M， n = 16）、(2)包被骨髓基质细胞（MC组，n = 16）、(3)与血管蒂结合（W组，n = 16）、(4)包被骨髓基质细胞并与血管蒂结合（MCV组，n = 16）。分别于2、4、6、8周测定各组4个种植体的孔内血管数量（血管孔比）和骨长入孔比例（骨孔比）。与对照组相比，当生物材料与血管蒂结合或包被成骨细胞时，血管生成更高。血管蒂和骨祖细胞的结合使血管化增加了60% (p = 0.003)，成骨增加了62% （p < 0.001）。血管蒂和骨髓骨祖细胞在珊瑚植入物中的结合，在异位肌内植入后，比单独使用任何一种都能更大程度地增强新生血管和成骨。
Research in biomaterials for bone reconstruction has led to elaborate osteogenic composites that combine porous ceramics with bone marrow stromal cells. The aim of this study was to evaluate the influence of direct vascularization of such composites on osteogenesis and the ability to produce a vascularized bone substitute transplant in an ectopic muscular site. Sixty-four coralline biomaterials were implanted in 32 Fisher rats under four conditions: (1) alone (reference group M, n = 16), (2) coated with bone marrow stromal cells (group MC, n = 16), (3) combined with a vascular pedicle (group W, n = 16), or (4) coated with bone marrow stromal cells and combined with a vascular pedicle (MCV group, n = 16). The number of vessels in the pores (vessel-pore ratio) of the implants and the proportion of pores showing bone ingrowth (bone-pore ratio) were measured at 2, 4, 6, and 8 weeks on four implants of each group. Compared with the reference group, angiogenesis was higher when the biomaterial was combined with a vascular pedicle or was coated with osteoprogenitor cells. The association of both vascular pedicle and osteoprogenitor cells increased vascularization by 60 percent (p = 0.003) and osteogenesis by 62 percent (p < 0.001). A combination of both vascular pedicle and bone marrow osteoprogenitor cells in coralline implants enhances neovascularization and osteogenesis after implantation in ectopic intramuscular sites to a greater extent than either does alone.