Over-expression of calcium-dependent protein kinase 13 and calreticulin interacting protein 1 confers cold tolerance on rice plants
Over-expression of calcium-dependent protein kinase 13 and calreticulin interacting protein 1 confers cold tolerance on rice plants
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DOI：
10.1007/s00438-007-0220-6
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发表时间：
2007-06-01
影响因子：
3.1
通讯作者：
Yamaguchi, Masayuki
中科院分区：
文献类型：
--
作者：
Komatsu, Setsuko;Yang, Guangxiao;Yamaguchi, Masayuki
钙是一种普遍存在的信号分子，细胞质钙离子浓度的变化参与植物对各种刺激的反应。水稻钙依赖蛋白激酶13（CDPK 13）和钙网蛋白相互作用蛋白1（CRTintP 1）参与水稻的冷胁迫反应。在这项研究中，用正义CDPK 13或CRTintP 1构建体转化的水稻品系被生产并用于研究这些蛋白质的功能。当植物在5 ℃下孵育3天时，正义转基因和载体对照水稻植物的叶片都变得萎蔫和卷曲。当植物在冷处理后被转移回非胁迫条件下时，正义转基因水稻植物的叶片死亡，但叶鞘保持绿色。在包括耐冷水稻品种在内的几个水稻品种中进一步检测了CDPK 13或CRTintP 1的表达。这些蛋白质在耐冷水稻品种中的积累高于耐冷水稻品种。为了研究过量表达CDPK 13和CRTintP 1是否会对蛋白质产生任何影响，使用蛋白质组学分析正义转基因水稻植株。将来自载体对照的蛋白质的2D-PAGE图谱与正义转基因水稻植物的蛋白质的2D-PAGE图谱进行比较。两种不同的蛋白质是钙网蛋白。结果表明，CDPK 13、钙网蛋白和CRTintP 1可能是水稻响应冷胁迫的重要信号组分。
Calcium is a ubiquitous signaling molecule and changes in cytosolic calcium concentration are involved in plant responses to various stimuli. The rice calcium-dependent protein kinase 13 (CDPK13) and calreticulin interacting protein 1 (CRTintP1) have previously been reported to be involved in cold stress response in rice. In this study, rice lines transformed with sense CDPK13 or CRTintP1 constructs were produced and used to investigate the function of these proteins. When the plants were incubated at 5 degrees C for 3 days, leaf blades of both the sense transgenic and vector control rice plants became wilted and curled. When the plants were transferred back to non-stress conditions after cold treatment, the leaf blades died, but the sheaths remained green in the sense transgenic rice plants. Expression of CDPK13 or CRTintP1 was further examined in several rice varieties including cold-tolerant rice varieties. Accumulation of these proteins in the cold-tolerant rice variety was higher than that in rice varieties that are intermediate in their cold tolerance. To examine whether over-expression of CDPK13 and CRTintP1 would have any effect on the proteins or not, sense transgenic rice plants were analyzed using proteomics. The 2D-PAGE profiles of proteins from the vector control were compared with those of the sense transgenic rice plants. Two of the proteins that differed between these lines were calreticulins. The results suggest that CDPK13, calreticulin and CRTintP1 might be important signaling components for response to cold stress in rice.