The ectonucleotidases alkaline phosphatase and nucleoside triphosphate diphosphohydrolas 2 are associated with subsets of progenitor cell populations in the mouse Embryonic, postnatal and adult neurogenic zones
The ectonucleotidases alkaline phosphatase and nucleoside triphosphate diphosphohydrolas 2 are associated with subsets of progenitor cell populations in the mouse Embryonic, postnatal and adult neurogenic zones
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DOI：
10.1016/j.neuroscience.2007.07.064
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发表时间：
2007-12-19
期刊：
影响因子：
3.3
通讯作者：
Zimmermann, H.
中科院分区：
文献类型：
--
作者：
Langer, D.;Ikehara, Y.;Zimmermann, H.
心室下区（SVZ）衍生的成人神经球表达两种外核苷酶，核苷三磷酸二磷酸水解酶2 （NTPDase2）和组织非特异性碱性磷酸酶（TNAP）。核苷酸受体P2Y(1)和P2Y(2)的激动剂以及腺苷增强了生长因子介导的祖细胞增殖。NTPDase2分别将ATP和UTP转化为ADP和UDP，它们都是P2Y受体激动剂。TNAP水解核苷三磷酸和二磷酸，并产生PI受体激动剂腺苷。在SVZ中，NTPDase2在B型细胞中特异性表达。为了进一步研究嘌呤能信号通路关键分子与神经源性区域的关系，我们分析了成年和发育中的小鼠脑侧脑室TNAP的表达。在成人大脑中，TNAP在SVZ的B型、A型和至少C型细胞亚群中表达，并在整个吻侧迁移流中表达。几乎100%的成年SVZ增殖的ki -67阳性细胞对TNAP染色，支持TNAP与SVZ祖细胞普遍存在关联的概念。相反，齿状回的ntpdase2阳性祖细胞是tnap阴性的。基本上所有胚胎日(E) 14的脑端囊泡细胞都显示TNAP活性，包括双皮质素阳性的神经母细胞。在胚胎进一步发育过程中，TNAP活性的增强仅限于心室和SVZ细胞。与TNAP相反，NTPDase2首先在围生期SVZ中表达，与TNAP阳性的SVZ边界细胞相关。在发育后期，ntpdase2阳性细胞从心室表面消失，并开始在心室下双皮质素阳性细胞簇周围形成鞘，明显转化为B型细胞。我们的研究结果确定了TNAP和NTPDase2是成年和发育中的小鼠大脑中祖细胞亚群的新标记物。他们进一步支持细胞外核苷酸和核苷信号传导有助于胚胎和成人神经发生的观点。(c) 2007年Elsevier Ltd代表IBRO出版。
Subventricular zone (SVZ)-derived adult neurospheres express two ectonucleotidases, nucleoside triphosphate diphosphohydrolase 2 (NTPDase2) and tissue nonspecific alkaline phosphatase (TNAP). Agonists of the nucleotide receptors P2Y(1) and P2y(2) as well as adenosine augment growth factor-mediated progenitor cell proliferation. NTPDase2 converts ATP and UTP to ADP and UDP, respectively, which are all P2Y receptor agonists. TNAP hydrolyzes nucleoside triphosphates and diphosphates and produces the PI receptor agonist adenosine. In the SVZ, NTPDase2 is specifically expressed by type B cells. In order to further scrutinize the association of key molecules of the purinergic signaling pathway with neurogenic regions, we analyzed the expression of TNAP at the lateral ventricles of the adult and developing mouse brain. In the adult brain, TNAP was expressed by type B, type A and at least subsets of type C cells of the SVZ and throughout the rostral migratory stream. Almost 100% of the proliferating, Ki-67-positive cells of the adult SVZ stained for TNAP, supporting the notion of a ubiquitous association of TNAP with SVZ progenitors. In contrast, NTPDase2-positive progenitors of the dentate gyrus were TNAP-negative. Essentially all cells of the telencephalic vesicle at embryonic day (E) 14 revealed TNAP activity, including doublecortin-positive neuroblasts. During further embryonic development, enhanced TNAP activity became restricted to cells of the ventricular and SVZ. In contrast to TNAP, NTPDase2 was first expressed in the SVZ perinatally, in association with TNAP-positive SVZ border cells. During later development, NTPDase2-positive cells disappeared from the ventricular surface and began to form sheaths around clusters of sub-ventricular doublecortin-positive cells, apparently transforming into type B cells. Our results identify TNAP and NTPDase2 as novel markers for subsets of progenitors in the adult and developing mouse brain. They further support the notion that signaling via extracellular nucleotides and nucleosides contributes to embryonic and adult neurogenesis. (c) 2007 Published by Elsevier Ltd on behalf of IBRO.