DISSOCIATION OF ALLERGENIC AND IMMUNOGENIC FUNCTIONS IN CONTACT SENSITIVITY TO PARA-PHENYLENEDIAMINE
DISSOCIATION OF ALLERGENIC AND IMMUNOGENIC FUNCTIONS IN CONTACT SENSITIVITY TO PARA-PHENYLENEDIAMINE
复制标题
DOI：
10.1159/000236573
复制
发表时间：
1993-01-01
影响因子：
2.8
通讯作者：
SCHMITT, D
中科院分区：
文献类型：
--
作者：
KRASTEVA, M;NICOLAS, JF;SCHMITT, D
对二苯甲二胺（PPD）的接触敏感性是一种频繁的延迟型超敏反应，导致接触性皮炎。本研究的目的是在16例对PPD过敏的患者中进行（通过阳性贴片测试评估）是为了更好地了解PPD接触敏感性中T细胞激活的机理。 PPD无法从第一组9例患者中诱导T细胞的显着增殖。在另外7例患者中，使用PPD和2个PPD代谢产物，即Bandrowski的碱（BB）和苯甲酰酮（BQ）评估淋巴细胞增殖。 BB在所有7例患者中以剂量依赖性的方式特异性刺激了T细胞增殖，而BQ（如PPD）都是无效的。 8 ppd非过敏个体的外周血单核细胞（PBMC）对PPD，BB或BQ没有反应。从这项研究中，我们得出结论：（1）PPD超敏反应中的免疫原性触觉本身不是PPD，并且（2）BB可能是具有T细胞激活特性的PPD的氧化衍生物。观察结果是，在PPD存在的情况下，源自PPD过敏患者的PBMC的T细胞系对BB的反应，但对PPD没有反应。我们的体外结果表明，PPD是一种prohapten，将其应用于皮肤上并将其代谢并转化为产物（例如BB），这是可以激活特定T细胞的免疫原性触觉。
Contact sensitivity to para-phenylenediamine (PPD) is a frequent delayed-type hypersensitivity resulting in contact dermatitis. The aim of the present study, conducted in 16 patients allergic to PPD (as assessed by a positive patch test), was to get better insight into the mechanism of T-cell activation in PPD contact sensitivity. PPD was unable to induce significant proliferation of T cells from a first set of 9 patients. In 7 further patients, lymphocyte proliferation was assessed using PPD and 2 PPD metabolites, namely Bandrowski's base (BB) and benzoquinone (BQ). BB specifically stimulated T-cell proliferation in a dose-dependent fashion in all 7 patients whereas BQ, like PPD, was ineffective. The peripheral blood mononuclear cells (PBMC) of 8 PPD nonallergic individuals did not respond to either PPD, BB or BQ. We concluded from this study that: (1) the immunogenic hapten in PPD hypersensitivity is not PPD itself, and (2) BB might be the oxidative derivative of PPD endowed with T-cell-activating properties. Further support to this statement was provided by the observation that a T cell line derived from PBMC of a PPD-allergic patient in the presence of PPD responded to BB but not to PPD. Our in vitro results suggest that PPD is a prohapten which when applied on the skin is metabolized and converted into products (such as BB) which are the immunogenic haptens able to activate specific T cells.