The restorer Rfo gene acts post-translationally on the stability of the ORF138 Ogura CMS-associated protein in reproductive tissues of rapeseed cybrids
The restorer Rfo gene acts post-translationally on the stability of the ORF138 Ogura CMS-associated protein in reproductive tissues of rapeseed cybrids
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DOI：
10.1023/a:1006223908044
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发表时间：
1999-07-01
影响因子：
5.1
通讯作者：
Budar, F
中科院分区：
文献类型：
--
作者：
Bellaoui, M;Grelon, M;Budar, F
利用原生质体融合技术，分析了恢复基因Rfo对Ogura细胞质雄性不育（CMS）相关ORF 138蛋白表达的影响。我们表明，在植物的基因组中的Rfo基因的存在下，减少了ORF138蛋白在花芽中的量，这种效果是最显着的花药在发育阶段时，不育表型正常表达。然而，orf138转录本的量不受Rfo基因在相同器官中相同阶段的影响。芽和花药的总多核糖体分析表明，orf138转录本在不育和恢复植株中以相同的效率翻译。从这些结果中，我们推断Rfo基因产物作用于ORF 138蛋白的翻译后稳定性，导致蛋白质积累的减少和生育力的恢复。
This paper describes the analysis of the effect of the restorer gene Rfo on the expression of the ORF138 protein associated with Ogura cytoplasmic male sterility (CMS) which has been engineered in rapeseed by protoplast fusion. We show that the presence of the Rfo gene in the genome of the plants decreases the amount of ORF138 protein in floral buds, this effect being the most dramatic in anthers at the stage of development when the sterile phenotype is normally expressed. However, the amount of orf138 transcripts is not affected by the Rfo gene in the same organs at the same stages. Total polysome analyses of buds and anthers show that the orf138 transcripts are translated with the same efficiency in sterile and restored plants. From these results we infer that the Rfo gene product acts on the post-translational stability of the ORF138 protein, leading to a decrease in the accumulation of the protein and a restoration of fertility.