OXYTOCIN IN HUMAN-PLASMA - CORRELATION WITH NEUROPHYSIN AND STIMULATION WITH ESTROGEN
OXYTOCIN IN HUMAN-PLASMA - CORRELATION WITH NEUROPHYSIN AND STIMULATION WITH ESTROGEN
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DOI：
10.1210/jcem-52-5-988
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发表时间：
1981-01-01
影响因子：
5.8
通讯作者：
ROBINSON, AG
中科院分区：
文献类型：
--
作者：
AMICO, JA;SEIF, SM;ROBINSON, AG
本文介绍了人血浆中催产素的放射免疫测定法。从血浆中较大的肽中提取催产素使用丙酮沉淀，具有75% ± 10%的纯度。2催产素的SEM [平均值的标准误差]回收率。测定的非特异性结合小于4%，最低检测水平为0.2 μ U/管。与垂体后叶素、精氨酸或赖氨酸加压素无交叉反应。平均基础水平（±.男性催产素的平均值为1.80 ± 0.001。0.07而在正常妇女中无差异（1.71 ± 0.01 μ U/ml）。0.07μ U/ml）。姿势的改变对催产素的水平没有影响。在4小时内每15分钟获得的样品显示没有催产素的脉动分泌。在长期接受雌激素作为口服避孕药的妇女中，催产素高于正常值（4.59 ± 0.01）。0.51μ U/ml; P < 0.01）。雌激素刺激的neurophysin也升高（8.45 ± 0.01）。1.99 ng/ml; P < 0.005）。急性摄入雌激素引起血浆中催产素水平的增加，12小时，并伴随着升高的雌激素刺激的neurophysin。当从摄入雌激素后从受试者获得的血浆中分离神经垂体后叶素时，来自血浆的神经垂体后叶素与雌激素刺激的神经垂体后叶素的人垂体标准品在聚丙烯酰胺凝胶上共迁移。在升高neurophysin水平的研究中，血浆中催产素水平与雌激素刺激的neurophysin水平之间存在显著相关性（P < 0.01）。雌激素给药刺激催产素和雌激素刺激的neurophysin的释放的观察提供了额外的证据，该neurophysin是人的催产素-neurophysin。
Radioimmunoassay for the measurement of oxytocin in human plasma is described. Extraction of oxytocin from larger peptides in plasma used acetone precipitation with a 75% .+-. 2 SEM [standard error of the mean] recovery of oxytocin. Nonspecific binding of the assay was less than 4%, and the minimum level of detection was 0.2 .mu.U/tube. No cross-reactivity was noted with neurophysins, arginine, or lysine vasopressin. The mean basal level (.+-. SEM) of oxytocin in men was 1.80 .+-. 0.07 .mu.U/ml and was not different in normal women (1.71 .+-. 0.07 .mu.U/ml). Changes in posture had no effect on the levels of oxytocin. Samples obtained every 15 min over 4 h showed no pulsatile secretion of oxytocin. In women chronically receiving estrogen as an oral contraceptive, oxytocin was greater than normal (4.59 .+-. 0.51 .mu.U/ml; P < 0.01). Estrogen-stimulated neurophysin was also elevated (8.45 .+-. 1.99 ng/ml; P < 0.005). Acute ingestion of estrogen caused an increase in the level of oxytocin in plasma by 12 h and a concomitant elevation of estrogen-stimulated neurophysin. When the neurophysin was isolated from plasma obtained from a subject after ingestion of estrogen, the neurophysin from plasma comigrated on a polyacrylamide gel with a human pituitary standard of estrogen-stimulated neurophysin. In the studies in which neurophysin was elevated, the correlation between the level of oxytocin and the level of estrogen-stimulated neurophysin in plasma was significant (P < 0.01). The observation that estrogen administration stimulates the release of oxytocin and estrogen-stimulated neurophysin provides additional evidence that this neurophysin is the oxytocin-neurophysin of man.