Accurate intracellular and in vivo temperature sensing based on CuInS2/ZnS QD micelles
Accurate intracellular and in vivo temperature sensing based on CuInS2/ZnS QD micelles
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基于 CuInS2/ZnS QD 胶束的精确细胞内和体内温度传感
DOI：
10.1039/c8tb03261k
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发表时间：
2019
影响因子：
7
通讯作者：
Wu Daocheng
中科院分区：
文献类型：
--
作者：
Zhang Hui;Wu Youshen;Gan Zhenhai;Yang Yuexuan;Liu Yiming;Tang Peng;Wu Daocheng
准确、灵敏的温度传感对于研究细胞和组织的生物活动具有重要意义。由于微环境的复杂性，现有的测温系统大多无法满足高灵敏度、低毒性的细胞内和体内温度精确传感的需求。经过研究，我们发现CuInS2/ZnS量子点（QD）的荧光强度明显依赖于温度，这些QD被两亲性胶束（QD-micelles）封装后可用于细胞内和体内温度传感。这些 QD 胶束的尺寸约为 10.1 ± 2.5 nm，发射带约为 650 nm，具有强烈的光致发光 (PL)。同时，其在接近室温下2%°C−1的高热灵敏度对溶液pH、离子强度和蛋白质浓度不敏感。即使浓度为 300 μg mL−1，我们的 QD 胶束也不会对 HeLa 和 PC-3 细胞产生细胞毒性作用。与分散在水中类似，QD-胶束在细胞内和肿瘤组织中继续表现出2.0%°C−1和2.1%°C−1的高热敏感性，并且在生理温度范围内的PL强度（I）和温度（T）具有良好的线性相关性。因此，它们在生物医学应用中具有巨大的潜力。
Accurate and sensitive temperature sensing is of great importance for studying biological activities in cells and tissues. Due to the complexity of the microenvironments, most of the existing thermometry systems cannot meet the needs of accurate intracellular and in vivo temperature sensing with high-sensitivity and low-toxicity. After investigation, we found that the fluorescence intensity of CuInS2/ZnS quantum dots (QDs) is significantly temperature dependent, and these QDs can be used for intracellular and in vivo temperature sensing after being encapsulated by amphiphilic micelles (QD-micelles). These QD-micelles have a size of approximately 10.1 ± 2.5 nm and an emission band of approximately 650 nm with intense photoluminescence (PL). Meanwhile, its high thermal sensitivity of 2% °C−1 at near-room temperature is insensitive to solution pH, ionic strength, and protein concentration. Even at a concentration of 300 μg mL−1, our QD-micelles do not exert cytotoxic effects on HeLa and PC-3 cells. Similar to being dispersed in water, the QD-micelles continue to exhibit a high thermal sensitivity of 2.0% °C−1 intracellular and 2.1% °C−1 in tumor tissues, and the PL intensity (I) and temperature (T) over the physiological temperature range have a good linear correlation. Therefore, they have considerable potential in biomedical applications.