Inhibition of 3,4-dihydroxy-L-phenylalanine decarboxylase in rat striatal synaptosomes by amino acids interacting with substrate transport.
Inhibition of 3,4-dihydroxy-L-phenylalanine decarboxylase in rat striatal synaptosomes by amino acids interacting with substrate transport.
复制标题
通过与底物转运相互作用的氨基酸抑制大鼠纹状体突触体中的 3,4-二羟基-L-苯丙氨酸脱羧酶。
DOI：
10.1016/0005-2736(80)90424-1
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发表时间：
1980
期刊：
影响因子：
--
通讯作者：
I. Katz
中科院分区：
文献类型：
--
作者：
I. Katz
在大鼠纹状体突触体中，由3，4-二羟基-L-苯丙氨酸合成多巴胺被一些具有芳香族或大脂肪族侧链的氨基酸抑制。在破坏的突触体中测量芳香族氨基酸脱羧酶活性时，未观察到抑制作用。类似地，在亮氨酸存在下观察到从酪氨酸合成多巴胺的抑制。这种抑制作用很可能是由于氨基酸与穿过突触体质膜的底物转运相互作用，而不是直接与催化酶作用。所获得的动力学数据被用来推断有关的运输过程的信息，他们建议的潜在重要性的氨基酸外排作为一个监管步骤。
Dopamine synthesis from 3,4-dihydroxy-l-phenylalanine in rat striatal synaptosomes was inhibited by a number of amino acids with aromatic or large aliphatic side chains. Inhibition was not seen when aromatic amino acid decarboxylase activity was measured in disrupted synaptosomes. Similarly, inhibition of dopamine synthesis from tyrosine was seen in the presence of leucine. The inhibition most likely results from interactions of the amino acids with substrate transport across the synaptosome plasma membrane, rather than directly with the catalytic enzymes. The kinetic data obtained are used to infer information about the relevant transport process; they suggest the potential importance of amino acid efflux as a regulatory step.