OBSERVATIONS ON THE ETIOLOGIC RELATIONSHIP OF ACHYLIA GASTRICA TO PERNICIOUS ANEMIA. VII. RESEMBLANCES BETWEEN THE PROTEOLYTIC ACTIVITY OF NORMAL HUMAN GASTRIC JUICE ON CASEIN IN NEUTRAL SOLUTION AND THE ACTIVITY OF THE INTRINSIC FACTOR.
OBSERVATIONS ON THE ETIOLOGIC RELATIONSHIP OF ACHYLIA GASTRICA TO PERNICIOUS ANEMIA. VII. RESEMBLANCES BETWEEN THE PROTEOLYTIC ACTIVITY OF NORMAL HUMAN GASTRIC JUICE ON CASEIN IN NEUTRAL SOLUTION AND THE ACTIVITY OF THE INTRINSIC FACTOR.
复制标题
胃膻病与恶性贫血病因关系的观察。
DOI：
10.1172/jci100958
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发表时间：
1938
影响因子：
15.9
通讯作者：
Margaret A. Adams
中科院分区：
文献类型：
--
作者：
F. H. Taylor;WilliamB. Castle;Robert W. Heinle;Margaret A. Adams
MethodsA 1 per cent solution of sodium caseinate was pre-pared as follows: 1 gram of washed casein (A. H. Thomas and Co.) was suspended in 20 ml. of water. Ten ml. of 2.5 N sodium hydroxide were added and the suspension shaken in order to effectsolution of the casein. Normal hydrochloric acid (approximately 22 ml.) was then added slowly to the point of incipient precipitation and a final adjustment to pH 7.4 was made with N/10 hydrochloric acid. Finally, the casein solution was diluted with distilled water to a volume of 100 ml.Fresh gastric juice or other preparations were brought to approximately pH 7.4 with normal sodium hydroxide solution and final adjustment to this pH was made with N/10 sodium hydroxide. Fifty ml. of such material were rapidly mixed inan Erlenmeyer flask with 50 ml. of the 1 per cent casein solution. The mixture was adjusted if necessaryto pH 7.4, and 2 ml. of toluol were added. The flasks were set in'a constanttemperature bath at 37.5 C. for 24 hours. The reaction of the digest at no time fell below pH 7. Plate cultures at the end of 24 hours were negative although occasionally turbidities appeared in broth cultures.
