Geographic variation in human mitochondrial DNA from Papua New Guinea.
Geographic variation in human mitochondrial DNA from Papua New Guinea.
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巴布亚新几内亚人类线粒体 DNA 的地理变异。
DOI：
10.1093/genetics/124.3.717
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发表时间：
1990
期刊：
影响因子：
3.3
通讯作者：
Wilson,AC
中科院分区：
文献类型：
--
作者：
Stoneking,M;Jorde,LB;Bhatia,K;Wilson,AC
高分辨率的线粒体DNA（mtDNA）限制性内切酶图谱，包括平均370个位点，从25个地点在巴布亚新几内亚（PNG）的119人。将这些PNG限制性内切酶图谱与已发表的来自澳大利亚、高加索、亚洲和非洲的mtDNA图谱进行比较，发现PNG具有最低量的mtDNA变异，并且PNG mtDNA谱系起源于东南亚。通过将观察到的GST值与随机重新分配数据产生的GST值分布进行比较，评估了种群mtDNA地理结构的统计学意义。这些分析表明，在世界各地的人口之间，高原和沿海巴布亚新几内亚人口之间，甚至两个高原巴布亚新几内亚人口之间相距约200公里的mtDNA变异有显着的结构。然而，巴布亚新几内亚沿海的种群基本上是随机繁殖的，尽管它们分布在数百公里之外。高原巴布亚新几内亚人口也有更多的mtDNA变异和更多的mtDNA类型代表每个创始血统比沿海巴布亚新几内亚人口。所有这些观察结果都与巴布亚新几内亚高地人口的更古老、有限的起源、巴布亚新几内亚高地人口彼此之间以及与沿海人口的内部隔离以及巴布亚新几内亚沿海人口的更近期和广泛的迁移相一致。一个明显的语言对巴布亚新几内亚的mtDNA变异的影响消失时，考虑到地理。高分辨率的mtDNA变异检测技术，再加上在一个单一的定义区域内广泛的地理采样，导致地理对mtDNA变异在人群中的影响有了更深入的了解。
High resolution mitochondrial DNA (mtDNA) restriction maps, consisting of an average of 370 sites per mtDNA map, were constructed for 119 people from 25 localities in Papua New Guinea (PNG). Comparison of these PNG restriction maps to published maps from Australian, Caucasian, Asian and African mtDNAs reveals that PNG has the lowest amount of mtDNA variation, and that PNG mtDNA lineages originated from Southeast Asia. The statistical significance of geographic structuring of populations with respect to mtDNA was assessed by comparing observed GST values to a distribution of GST values generated by random resampling of the data. These analyses show that there is significant structuring of mtDNA variation among worldwide populations, between highland and coastal PNG populations, and even between two highland PNG populations located approximately 200 km apart. However, coastal PNG populations are essentially panmictic, despite being spread over several hundred kilometers. Highland PNG populations also have more mtDNA variability and more mtDNA types represented per founding lineage than coastal PNG populations. All of these observations are consistent with a more ancient, restricted origin of highland PNG populations, internal isolation of highland PNG populations from one another and from coastal populations, and more recent and extensive population movements through coastal PNG. An apparent linguistic effect on PNG mtDNA variation disappeared when geography was taken into account. The high resolution technique for examining mtDNA variation, coupled with extensive geographic sampling within a single defined area, leads to an enhanced understanding of the influence of geography on mtDNA variation in human populations.