Comparison of metastatic brain tumour models using three different methods: the morphological role of the pia mater
Comparison of metastatic brain tumour models using three different methods: the morphological role of the pia mater
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DOI：
10.1111/j.1365-2613.2007.00563.x
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发表时间：
2008-02-01
影响因子：
3
通讯作者：
Ueda, Morikazu
中科院分区：
文献类型：
--
作者：
Saito, Norihiko;Hatori, Tsutomu;Ueda, Morikazu
随着癌症诊断和治疗方法的进步，人们对转移性脑肿瘤的兴趣不断增加。有许多研究使用了不同的动物模型方法，我们认为每种模型反映了不同的病理过程，因为大脑的独特组成。我们用三种不同的方法制备了转移性脑瘤模型。在这项研究中，我们试图阐明软脑膜在脑转移中的作用。转移灶呈血管中心型，形成肿瘤细胞环，被命名为血管周围增生性病变。此外，我们观察到肿瘤细胞渗入脑实质，其边界变得模糊。这些都被贴上了“侵袭性增殖”的标签。颈内动脉注射模型反映了血源性转移。在该模型中，血管周围和侵袭性增殖均可观察到。鞘内注射模型反映了软脑膜癌病。在这个模型中，观察到脑膜转移。在立体定向注射模型中，观察到注射部位肿瘤的增殖和向脑实质的侵袭。当肿瘤细胞扩散到血管周围空间形成血管中心型时，软胶质膜起支架作用。软脑胶质膜位于脑实质和血管之间。血管通过被软膜覆盖的被称为血管周围空间的隧道穿透大脑。我们制备的三种不同的方法反映了三种不同的病理过程。我们的发现表明软脑膜是脑转移的关键因素。
As methods of cancer diagnosis and treatment progress, interest in metastatic brain tumours continues to increase. There are many studies using various methods of animal model and we considered that each model reflects different pathological processes because of the unique composition of the brain. We prepared metastatic brain tumour models using three different methods. In this study, we attempted to elucidate the roles of the pia mater in brain metastasis. The metastatic foci showed an angiocentric pattern, forming collars of neoplastic cells, and were designated 'perivascular proliferations'. Furthermore, we observed neoplastic cells that infiltrated the brain parenchyma, the border of which had become indistinct. These were labelled 'invasive proliferations'. The internal carotid artery injection model reflects haematogenous metastasis. In this model, both perivascular and invasive proliferations were observed. The intrathecal injection model reflects leptomeningeal carcinomatosis. In this model, metastasis to the meninges was observed. In the stereotactic injection model, the tumour proliferation at the injection site and the infiltration into the brain parenchyma were observed. The pia-glial membrane serves as a scaffold when neoplastic cells spread to the perivascular space forming angiocentric pattern. The pia-glial membrane is found between the brain parenchyma and blood vessels. Blood vessels penetrate the brain through tunnels known as perivascular spaces that are covered by pia mater. Three different methods which we prepared reflect three different pathological processes. Our findings suggest that the pia mater is a critical factor in brain metastasis.