Experimental studies on preparation of the porous and small-diameter poly(ε-caprolactone) external vascular scaffold and its degradability and biocompatibility.
Experimental studies on preparation of the porous and small-diameter poly(ε-caprolactone) external vascular scaffold and its degradability and biocompatibility.
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DOI：
10.1051/rmr/180001
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发表时间：
2018
影响因子：
2
通讯作者：
Feng L
中科院分区：
文献类型：
--
作者：
Chen Q;Jiang X;Feng L
目的：本研究采用浸渍浸提法制备多孔聚己内酯（PCL）生物可降解血管外支架，并对其力学性能、降解性能和生物相容性进行评价。研究方法：以PCL-1、PCL-2为原料，NaCl颗粒为致孔剂，构建多孔PCL体外血管支架。测试了多孔PCL体外血管支架的力学性能。研究了该支架在嗜热菌脂肪酶（TL脂肪酶）存在下的降解性能。在1、3和5、7天后，取出样品，并测量培养基的pH。通过宏观观察和扫描电镜观察支架的微观结构，考察支架的形态稳定性。CCK-8法检测支架的细胞毒性。结果：PCL-1可制备出质地均匀的白色完整血管外支架。当NaCl浓度小于或等于50%时，多孔PCL-1血管外支架的拉伸强度大于4.2Mpa，满足临床血管移植的要求。随着支架在脂肪酶介质中的降解，支架的形状稳定性被严重破坏，支架表面开始降解，出现蜂窝状孔洞，介质的pH值低于初始阅读7.4。与对照组相比，大鼠脂肪干细胞（rADSCs）在多孔PCL外血管支架的提取物中培养具有良好的增殖和细胞形态。结论：当NaCl浓度为50%时，多孔PCL-1-50血管外支架在具有足够机械强度的基础上孔隙率最大，满足临床血管移植的要求。该支架与rADSCs具有良好的生物相容性，其降解机制为表面降解。
Aim: This study was aim to prepare a porous poly(ε-caprolactone) (PCL) biodegradable external vascular scaffold by dipping and leaching method, and to assess its mechanical property, degradability and biocompatibility. Methods: We used the PCL-1, PCL-2 as the raw materials and NaCl particles as the pore-forming agents to construct a porous PCL external vascular scaffold. We tested the mechanical property of the porous PCL external vascular scaffold. The degradability of the scaffold was studied in the presence of thermomyces lanuginosus lipase (TL lipase). After 1, 3, and 5, 7 days, the samples were taken out, and the pH of the media was measured. The form-stability of the scaffold was investigated by macroscopic observation and the microstructure of it was observed by SEM. The cytotoxicity of the scaffold was evaluated by CCK-8 assay. Results: PCL-1 could make a white integrated external vascular scaffold with uniform texture. When the concentration of NaCl was less than or equal to 50%, the tensile strength of the porous PCL-1 external vascular scaffolds were higher than 4.2 Mpa, which meet the demand of clinical vascular transplantation. With the degradation of the scaffold in the lipase media, the form-stability of the scaffold was seriously destroyed, the surface of the scaffold began to degrade with some honeycomb holes, and the pH of the media values were lower than the initial reading of 7.4. Rat adipose-derived stem cells (rADSCs) cultured in the extractions of the porous PCL external vascular scaffold had good proliferation and cell morphology compared to the control group. Conclusion: The porous PCL-1-50 external vascular scaffold, with the 50% concentration of NaCl, had the maximum porosity on the basis of enough mechanical strength which meets the demand of clinical vascular transplantation. Moreover, it had good biocompatibility with rADSCs and the degradation mechanism of the scaffold was surface degradation.