Two VOZ transcription factors link an E3 ligase and an NLR immune receptor to modulate immunity in rice
Two VOZ transcription factors link an E3 ligase and an NLR immune receptor to modulate immunity in rice
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两个 VOZ 转录因子连接 E3 连接酶和 NLR 免疫受体以调节水稻的免疫力
DOI：
10.1016/j.molp.2020.11.005
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发表时间：
2021
期刊：
影响因子：
27.5
通讯作者：
Wang Guo-Liang
中科院分区：
文献类型：
--
作者：
Wang Jiyang;Wang Ruyi;Fang Hong;Zhang Chongyang;Zhang Fan;Hao Zeyun;You Xiaoman;Shi Xuetao;Park Chan Ho;Hua Kangyu;He Feng;Bellizzi Maria;Vo Kieu Thi Xuan;Jeon Jong-Seong;Ning Yuese;Wang Guo-Liang
核苷酸结合的富含亮氨酸重复序列(NLR)蛋白在植物免疫中发挥着重要作用。然而，NLR是如何被调控和激活防御信号的还没有完全弄清楚。水稻NLR受体Piz-t对稻瘟病菌具有广谱抗性，RING型E3连接酶AVRPIZ-T相互作用蛋白10(APIP10)负调控Piz-t的积累。在本研究中，我们发现APIP10与两个水稻转录因子，维管植物ONEZINC Finger 1(OsVOZ1)和OsVOZ2相互作用，并通过26S蛋白酶体途径促进它们的降解。OsVOZ1具有转录抑制活性，而OsVOZ2具有转录激活活性。在非Piz-t背景下，osvoz1和osvoz2单一突变体表现出对稻瘟病菌的轻微抗性，但相反。然而，osvoz1 osvoz2双突变体表现出强烈的矮化和细胞死亡，通过RNA干扰沉默这两个基因也会导致矮化，轻微的细胞死亡，并在非Piz-t背景下增强对稻瘟病菌的抗性。OsVOZ1和OsVOZ2都与Piz-t相互作用。在Piz-t背景下，OsVOZ1和OsVOZ2的双沉默降低了Piz-t蛋白的积累和转录，降低了活性氧依赖的细胞死亡，并降低了对含有AvrPiz-t的稻瘟病菌的抗性。综上所述，这些结果表明，OsVOZ1和OsVOZ2负向调节基础防御，而对PIZ-t介导的免疫起正作用。
Nucleotide-binding leucine-rich repeat (NLR) proteins play critical roles in plant immunity. However, how NLRs are regulated and activate defense signaling is not fully understood. The rice (Oryza sativa) NLR receptor Piz-t confers broad-spectrumresistance to the fungal pathogen Magnaporthe oryzae and the RINGtype E3 ligase AVRPIZ-T INTERACTING PROTEIN 10 (APIP10) negatively regulates Piz-t accumulation. In this study, we found that APIP10 interacts with two rice transcription factors, VASCULAR PLANT ONEZINC FINGER 1 (OsVOZ1) and OsVOZ2, and promotes their degradation through the 26S proteasome pathway. OsVOZ1 displays transcriptional repression activity while OsVOZ2 confers transcriptional activation activity in planta. The osvoz1 and osvoz2 single mutants display modest but opposite M. oryzae resistance in the non-Piz-t background. However, the osvoz1 osvoz2 double mutant exhibits strong dwarfism and cell death, and silencing of both genes via RNA interference also leads to dwarfism, mild cell death, and enhanced resistance to M. oryzae in the non-Piz-t background. Both OsVOZ1 and OsVOZ2 interact with Piz-t. Double silencing of OsVOZ1 and OsVOZ2 in the Piz-t background decreases Piz-t protein accumulation and transcription, reactive oxygen species-dependent cell death, and resistance to M. oryzae containing AvrPiz-t. Taken together, these results indicate that OsVOZ1 and OsVOZ2 negatively regulate basal defense but contribute positively to Piz-t-mediated immunity.