Effects of different open cryo-carriers on embryo survival and clinical outcome in frozen embryo transfer cycle patients
Effects of different open cryo-carriers on embryo survival and clinical outcome in frozen embryo transfer cycle patients
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不同开放式冷冻载体对冷冻胚胎移植周期患者胚胎存活和临床结局的影响
DOI：
10.1080/19396368.2017.1419510
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发表时间：
2018-01-01
影响因子：
2.4
通讯作者：
Li, Yuan
中科院分区：
文献类型：
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作者：
Chu, Dapeng;Fu, Lei;Li, Yuan
本研究的目的是比较不同的开放式冷冻载体：CryoloopTM、CryotopTM和CryoleafTM对冷冻胚胎移植（FET）周期患者胚胎存活和临床结果的影响。我们分析了325例使用CryoloopTM（160个周期）、CryotopTM（105个周期）或CryoleafTM（83个周期）玻璃化的348个FET周期患者的胚胎存活率和临床结果。3组胚胎存活率（98.8% vs 100% vs 97.7%, p>0.05）、HCG阳性率（58.8% vs 63.8% vs 57.8%, p>0.05）、生化妊娠率（6.9% vs 11.4% vs 9.6%, p>0.05）、着床率（33.2% vs 37.4% vs 34.1%, p>0.05）差异均无统计学意义。CryoloopTM组早期流产率显著高于crytoptm和CryoleafTM组（27.1%比3.6%和7.5%,p0.05）。本研究未发现新生儿畸形病例。总之，从临床结果和婴儿特点来看，CryotopTM和CryoleafTM在卵裂期和囊胚期都是有效的胚胎玻璃化。然而，CryoloopTM导致HCG阳性率降低，早期流产率增加，在卵裂期增加。缩写：LN2：液氮；注册会计师:冷冻保护剂;ART：辅助生殖技术；IVF：体外受精；胞浆内单精子注射；BMI：身体质量指数；FSH：卵泡刺激激素；COH：控制性卵巢过度刺激；FET：冷冻胚胎移植；mm:毫米;HCG：人绒毛膜促性腺激素；RCT：随机临床试验；NC：自然循环；AC：人工循环；EM：平衡介质；DMSO：二甲基亚砜；乙二醇；VM：玻璃化介质；WM：加热介质
The purpose of this study was to compare the efficacy of different open cryo-carriers: the CryoloopTM, CryotopTM, and CryoleafTM, in embryo survival and clinical outcome in patients with frozen embryo transfer (FET) cycle. We analyzed the embryo survival rate and clinical outcome in 325 patients of 348 FET cycles vitrified with the CryoloopTM (160 cycles), CryotopTM (105 cycles), or CryoleafTM (83 cycles). No significant differences were observed in embryo survival rate (98.8% vs. 100% vs. 97.7%, p>0.05), HCG positive rate (58.8% vs. 63.8% vs. 57.8%, p>0.05), biochemical pregnancy rate (6.9% vs. 11.4% vs. 9.6%, p>0.05), or implantation rate (33.2% vs. 37.4% vs. 34.1%, p>0.05) in the three groups respectively. The early abortion rate of the CryoloopTM group was significantly higher than that of the CryotopTM and CryoleafTM group (27.1% vs. 3.6% and 7.5%, p0.05). No case of neonatal malformation was observed in the present study. Overall, CryotopTM and CryoleafTM were effective for embryo vitrification at both the cleavage and blastocyst stage according to the results of clinical outcome and infant characteristics. However, CryoloopTM led to a decreased positive HCG rate and increased early abortion rate, heightened at the cleavage stage.Abbreviations: LN2: liquid nitrogen; CPA: cryoprotectant; ART: assisted reproductive technology; IVF: in vitro fertilization; ICSI: intracytoplasmic sperm injection; BMI: body mass index; FSH: follicular stimulation hormone; COH: controlled ovarian hyperstimulation; FET: frozen embryo transfer; mm: millimeter; HCG: human chorionic gonadotropin; RCT: randomized clinical trial; NC: natural cycle; AC: artificial cycle; EM: equilibration medium; DMSO: dimethyl sulphoxide; EG: ethylene glycol; VM: vitrification medium; WM: warming medium