Suppressor Mutants: History and Today's Applications.
Suppressor Mutants: History and Today's Applications.
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抑制突变体：历史和今天的应用。
DOI：
10.1128/ecosalplus.esp-0037-2020
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发表时间：
2021-12-15
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几十年来，生物学家利用分子和遗传工具以及分析为我们理解生物系统的能力提供的近乎无限的优势。这些遗传工具之一，抑制基因分析，已被证明是非常宝贵的，在进一步了解我们的生物过程和途径，并在发现未知的基因和基因产物之间的相互作用。抑制子分析的力量在于它能够以公正的方式发现遗传相互作用，经常导致令人惊讶的发现。随着技术的进步，高通量方法有助于大规模鉴定抑制因子，并有助于深入了解抑制因子发挥作用的核心功能机制。在这篇综述中，我们研究了一些基本的发现，已成为可能，通过分析抑制基因突变。此外，我们还介绍了可以分离的不同类型的抑制突变体，以及每种类型所提供的生物学见解。此外，我们提供的实验设计，以隔离抑制突变体和策略，以确定基因间抑制突变的考虑。最后，我们提供了抑制突变体的分离和映射的指导和示例协议。
For decades, biologist have exploited the near boundless advantages that molecular and genetic tools and analysis provide for our ability to understand biological systems. One of these genetic tools, suppressor analysis, has proven invaluable in furthering our understanding of biological processes and pathways and in discovering unknown interactions between genes and gene products. The power of suppressor analysis lies in its ability to discover genetic interactions in an unbiased manner, often leading to surprising discoveries. With advancements in technology, high-throughput approaches have aided in large-scale identification of suppressors and have helped provide insight into the core functional mechanisms through which suppressors act. In this review, we examine some of the fundamental discoveries that have been made possible through analysis of suppressor mutations. In addition, we cover the different types of suppressor mutants that can be isolated and the biological insights afforded by each type. Moreover, we provide considerations for the design of experiments to isolate suppressor mutants and for strategies to identify intergenic suppressor mutations. Finally, we provide guidance and example protocols for the isolation and mapping of suppressor mutants.