Compressed collagen gel as the scaffold for skin engineering
Compressed collagen gel as the scaffold for skin engineering
复制标题
压缩胶原凝胶作为皮肤工程的支架
DOI：
10.1007/s10544-010-9415-4
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发表时间：
2010-08-01
影响因子：
2.8
通讯作者：
Liu, Wei
中科院分区：
文献类型：
--
作者：
Hu, Kuikui;Shi, Hui;Liu, Wei
Collagen gel scaffolds can potentially be utilized as cell seeded systems for skin tissue engineering. However, its dramatic contraction after being mixed with cells and its mechanical weakness are the drawbacks for its application to skin engineering. In this study, a compressed collagen gel scaffold was fabricated through the rapid expulsion of liquid from reconstituted gels by the application of ‘plastic compression’(PC) technique. Both compressed and uncompressed gels were characterized with their gel contraction rate, morphology, the viability of seeded cells, their mechanical properties and the feasibility as a scaffold for constructing tissue-engineered skin. The results showed that the compression could significantly reduce the contraction of the collagen gel and improve its mechanical property. In addition, seeded dermal fibroblasts survived well in the compressed gel and seeded epidermal cells gradually developed into a stratified epidermal layer, and thus formed tissue engineered skin. This study reveals the potential of using compressed collagen gel as a scaffold for skin engineering.
