A novel permalloy based magnetic single cell micro array
A novel permalloy based magnetic single cell micro array
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DOI：
10.1039/b821044f
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发表时间：
2009-01-01
期刊：
影响因子：
6.1
通讯作者：
Park, Edward J.
中科院分区：
文献类型：
--
作者：
Liu, William;Dechev, Nikolai;Park, Edward J.
能够自动将细胞排列到几何阵列上的设备引起了生物医学研究人员的极大兴趣。这样的设备可以促进对许多细胞的研究，同时细胞保持彼此物理分离。通过这种方式，细胞阵列减少了细胞与细胞之间的相互作用，同时细胞都受到共同的刺激，这使得单个细胞的行为得以揭示。阵列的使用允许单细胞的并行分析，便于数据记录，并打开了使用基于自动化机器的单细胞分析技术的大门。提出了一种新型的坡莫合金基磁性单细胞微阵列（MSCMA）。MSCMA通过在预定义位置处产生磁通密度峰值来创建磁阱阵列。当使用用免疫磁性标记物标记的细胞时，细胞将与磁场相互作用，并且可以在磁性捕获位点处被捕获。MSCMA的原型已经成功地制造和测试使用固定和活Jurkat细胞（10毫米平均直径），标记。原型在实验性试验中表现如预测的那样。实验结果表明，MSCMA可以随机阵列高达136个单细胞每平方毫米。结果还表明，捕获的单细胞的数量是一个函数的MSCMA设计的陷阱网站密度和流体样品中的细胞密度。
Devices capable of automatically aligning cells onto geometrical arrays are of great interest to biomedical researchers. Such devices can facilitate the study of numerous cells while the cells remain physically separated from one another. In this way, cell arrays reduce cell-to-cell interactions while the cells are all subjected to common stimuli, which allows individual cell behaviour to be revealed. The use of arrays allows for the parallel analysis of single cells, facilitates data logging, and opens the door to the use of automated machine-based single cell analysis techniques. A novel permalloy based magnetic single cell micro array (MSCMA) is presented in this paper. The MSCMA creates an array of magnetic traps by generating magnetic flux density peaks at predefined locations. When using cells labelled with immunomagnetic labels, the cells will interact with the magnetic fields, and can be captured at the magnetic trap sites. Prototypes of the MSCMA have been successfully fabricated and tested using both fixed and live Jurkat cells (10 mm average diameter) that were labelled. The prototypes performed as predicted during experimental trials. The experimental results show that the MSCMA can randomly array up to 136 single cells per square mm. The results also show that the number of single cells captured is a function of the trap site density of the MSCMA design and the cell density in the fluid sample.