Isolation and application of a wild strain photosynthetic bacterium to environmental waste management
Isolation and application of a wild strain photosynthetic bacterium to environmental waste management
复制标题
一株野生光合细菌的分离及其在环境废物治理中的应用
DOI：
10.1007/bf03326237
复制
发表时间：
2011-06
影响因子：
3.1
通讯作者：
Zhang, G.
中科院分区：
文献类型：
--
作者：
Madukasi, E. I.;Chunhua, H.;Zhang, G.
经形态学鉴定，一株新的光合细菌为革兰氏阴性杆菌。该菌在光养条件下产生暗红色培养物，以出芽方式繁殖，形成平行于细胞质膜的层状胞质膜系统，内含细菌叶绿素a和类胡萝卜素。它的生理和营养需求测试表明，分离物在不同的环境条件下茁壮成长和繁殖。经16 SrDNA鉴定，Z 08为球形红细菌（Rhodobacter sphaeroides）。Z 08对大豆废水和制药废水的生物降解适应性试验表明，Z 08具有良好的废水生物修复能力。在3500 lx光照下，Z 08在悬浮式间歇式光生物反应器中处理制药废水的适应性最好，当废水稀释度为1：4时，在最短水力停留时间为3d时，化学需氧量降低了50%，生物量和比生长分别为780 mg/L和0.015/h。此外，处理和原料制药废水的气相色谱-质谱分析表明，制药废水中发现的高分子量的难降解化合物通过生物降解转化为毒性较小和可接受的低分子量物质。在自然光照条件下，Z 08处理大豆废水，化学需氧量、生物量和比生长速率分别降低了80%，达到1540 mg/L和0.025/h，而不考虑食物与微生物的比例。初步研究表明，Z 08菌株具有一定的毒性耐受能力，能够对高浓度有机废水中的难降解物质进行解毒，具有很大的细胞蛋白回收潜力。因此，菌株Z 08可用于污染废水流的生物降解。
A new photosynthetic bacterium isolate was morphologically identified as a non-motile rod-shape gram-negative bacterium. It produced a dark red culture under phototrophic condition, reproduced by budding and formed a lamellar intracytoplasmic membrane system parallel to cytoplasmic membrane, which contained bacteriochlorophyll a and caratenoids. It’s physiological and nutrient requirement tests gave indication that the isolate thrived and multiplied in varied environmental conditions. It was consequently named Z08 and identified as Rhodobacter sphaeroides by 16SrDNA. Adaptation of Z08 to biodegradation of two environmentally concerned wastewaters, i.e. soybean and pharmaceutical wastewaters, attested its potential in wastewater bioremediation. Z08 adaptation in a suspended batch photobioreactor treating pharmaceutical wastewater at 3500lx radiation recorded best result after wastewater dilution of 1:4 with concomitant chemical oxygen demand reduction, biomass yield and specific growth of 50 %, 780 mg/L and 0.015/h, respectively at the lowest hydraulic retention time of three days. Furthermore, gas chromatography mass spectra analyses of treated and raw pharmaceutical wastewater indicated that high molecular weight recalcitrant compounds found in the pharmaceutical wastewater were transformed to less toxic and acceptable lower molecular weight substances through biodegradation. Whilst Z08 treatment of soybean wastewater under natural light intensity radiation recorded 80 % reduction, 1540 mg/L and 0.025/h for chemical oxygen demand, biomass and specific growth rate respectively regardless of the food to microorganism ratio. This preliminary investigation showed that isolate Z08 has some toxic tolerance level which could detoxify refractory substances with great potential for cell protein recovery in high organic strength wastewater. Therefore, strain Z08 could be employed in biodegradation of contaminated wastewater streams.
登录
查看更多内容
DOI：
10.1007/bf03326128
发表时间：
2010-03
影响因子：
3.1
作者：
E. I. Madukasi;X. Dai;C. He;J. Zhou
通讯作者：
E. I. Madukasi;X. Dai;C. He;J. Zhou
DOI：
--
发表时间：
1982
期刊：
Impact of science on society
影响因子：
--
作者：
E. Dasilva
通讯作者：
E. Dasilva
影响因子：
1.5
作者：
A. Ventosa;E. Quesada;F. Rodríguez-Valera;F. Ruiz-Berraquero;A. Ramos‐Cormenzana
通讯作者：
A. Ventosa;E. Quesada;F. Rodríguez-Valera;F. Ruiz-Berraquero;A. Ramos‐Cormenzana
DOI：
10.1007/bf03326061
发表时间：
2009-12
影响因子：
3.1
作者：
X. X. Zhang-X.;D. Zhao;Z. X. Wang;Bo Wu;W. Li;S. Cheng
通讯作者：
X. X. Zhang-X.;D. Zhao;Z. X. Wang;Bo Wu;W. Li;S. Cheng
影响因子：
13.6
作者：
Yang, Yu;Wang, Peng;Liu, Yuan
通讯作者：
Liu, Yuan