Mg-Fe layered double hydroxides modified titanium enhanced the adhesion of human gingival fibroblasts through regulation of local pH level
Mg-Fe layered double hydroxides modified titanium enhanced the adhesion of human gingival fibroblasts through regulation of local pH level
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Mg-Fe层状双氢氧化物改性钛通过调节局部pH值增强人牙龈成纤维细胞的粘附
DOI：
10.1016/j.msec.2021.112485
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发表时间：
2021
期刊：
影响因子：
--
通讯作者：
Wang Donghui
中科院分区：
文献类型：
--
作者：
Yin Yijia;Jian Linjia;Li Baoe;Liang Chunyong;Han Xianglong;Zhao Xuefeng;Wang Donghui
牙种植体的耐久性与骨结合和周围软组织封闭密切相关。适当的局部pH值有利于成纤维细胞粘附并有助于软组织凝闭。层状双氢氧化物（LDHs）的特点是可调的碱度，提供了一种可能性，以研究pH值对细胞行为的影响。在此，我们制备了Mg单键Fe LDHs改性钛。在煅烧过程中，LDHs的局部pH值升高，而不会通过OH-交换机制改变其他物理和化学性质。体外研究表明，在250 °C下煅烧2 h的LDHs膜可提供10.17的局部pH，通过形成黏着斑复合物和激活黏着斑激酶相关信号通路，促进人牙龈成纤维细胞（hGFs）的早期粘附、增殖和I型胶原表达。总之，通过Mg单键Fe LDHs薄膜赋予钛表面适当的碱性，增强了hGFs的粘附性，为设计用于牙种植体周围软组织封闭的多功能生物材料提供了一种新的策略。
The durability of dental implants is closely related to osseointegration and surrounding soft tissue sealing. Appropriate local pH favors fibroblasts adhesion and contributes to soft tissue sealing. Layered double hydroxides (LDHs) are characterized by adjustable alkalinity, offering a possibility to investigate the influence of pH on cellular behaviors. Herein, we fabricated Mgsingle bondFe LDHs modified titanium. During calcination, the local pH value of LDHs increase, without altering other physics and chemical properties via OH−exchange mechanism. In vitro studies showed that LDHs films calcined at 250 °C for 2 h provide a local pH of 10.17, which promote early adhesion, proliferation, and type I collagen expression of human gingival fibroblasts (hGFs) through the formation of focal adhesion complex and activation of focal adhesion kinase related signaling pathways. In conclusion, endowing the titanium surface with appropriate alkalinity by Mgsingle bondFe LDHs films enhances the adhesion of hGFs, providing a new strategy of designing multifunctional biomaterials for soft tissue sealing around dental implants.