IgA immunity in HIV type 1-infected chimpanzees. I. Systemic immunity.
IgA immunity in HIV type 1-infected chimpanzees. I. Systemic immunity.
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1 型 HIV 感染黑猩猩的 IgA 免疫力。
DOI：
10.1089/aid.1997.13.1263
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发表时间：
1997
期刊：
影响因子：
--
通讯作者：
Jackson,S
中科院分区：
文献类型：
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作者：
Black,KP;Fultz,PN;Girard,M;Jackson,S
人类感染HIV会引起细胞和体液免疫系统的各种异常，包括B淋巴细胞的功能异常。在许多情况下，血清IgA调节功能障碍，导致这种免疫球蛋白同种类型的浓度升高。为了确定感染HIV-1的黑猩猩是否会出现与在人类中观察到的类似的IgA异常，我们量化了从六只感染HIV的黑猩猩和一只未感染的对照动物纵向收集的血清中总的IgA、Ig G和Ig M水平。与未感染动物的免疫球蛋白水平相比，六只受感染的黑猩猩中有两只在感染艾滋病毒后血清免疫球蛋白水平升高。另外两只受感染的动物在暴露于艾滋病毒后的10个月内，三种同工酶显著下降，随后恢复到基线水平。其余两只感染艾滋病毒的黑猩猩的血清免疫球蛋白水平与基线水平平行，在感染后20至45个月期间没有表现出很大的偏差。对免疫球蛋白A亚类的酶联免疫吸附试验分析显示，在对HIV-1没有表现出不规则的免疫球蛋白A、免疫球蛋白或免疫球蛋白M反应的两只动物中，可能存在免疫球蛋白A2亚类的异常。用酶免疫分析试剂盒(EIA)和针对env、Gag和Polgene产物的IgA、IgA1和IgA2抗体进行Western印迹分析，检测针对HIV抗原的特异性Ig G、Ig A、Ig A1和Ig A2抗体。由于免疫球蛋白可以掩盖在感染人类中检测到的HIV特异性IgA抗体，因此也对免疫球蛋白缺失的黑猩猩血清进行了蛋白质印迹和EIA检测。结果表明，在某些情况下，去除免疫球蛋白可以增强免疫球蛋白A对HIV抗原的反应性。这项研究表明，HIV-1能够诱导黑猩猩血清IgA表达异常。这些结果可能会进一步了解艾滋病毒如何影响感染者的体液反应。
HIV infection in humans causes various aberrancies in both the cellular and humoral immune systems, including functional abnormalities of B lymphocytes. In many instances, dysfunction occurs in the regulation of serum IgA, resulting in elevated concentrations of this immunoglobulin isotype. To determine whether HIV-1-infected chimpanzees develop IgA abnormalities similar to those observed in humans, we quantified total IgA, IgG, and IgM levels in sera collected longitudinally from six HIV-infected chimpanzees and one uninfected control animal. In comparison to immunoglobulin levels in the uninfected animal, two of the six infected chimpanzees exhibited increases in serum immunoglobulins following infection with HIV. Two other infected animals showed a marked decrease in the three isotypes within 10 months of exposure to HIV, followed by a return to baseline levels. The remaining two HIV-infected chimpanzees displayed serum immunoglobulin levels that paralleled the baseline levels and did not show great deviation over a period of 20 to 45 months postinfection. ELISA analyses of the IgA subclasses revealed possible abnormalities of the IgA2 subclass within the two animals that did not display irregular IgA, IgG, or IgM responses to HIV-1. Specific IgG, IgA, IgA1, and IgA2 antibodies to HIV antigens were detected by an enzyme immunoassay (EIA) kit and by Western blot analysis with IgA, IgA1, and IgA2 antibodies directed against theenv, gag, andpolgene products. Because IgG can mask the detection of HIV-specific IgA antibodies in infected humans, Western blots and EIAs were also performed on IgG-depleted chimpanzee sera. The results demonstrated that in some instances, IgA reactivity against HIV antigens can be enhanced on removal of IgG. This study indicates that HIV-1 is capable of inducing abnormalities in serum IgA expression in chimpanzees. These results might further understanding of how HIV affects humoral responses in infected humans.