Microbial composition of supra- and subgingival plaque in subjects with adult periodontitis
Microbial composition of supra- and subgingival plaque in subjects with adult periodontitis
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DOI：
10.1034/j.1600-051x.2000.027010722.x
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发表时间：
2000-10-01
影响因子：
6.7
通讯作者：
Socransky, SS
中科院分区：
文献类型：
--
作者：
Ximénez-Fyvie, LA;Haffajee, AD;Socransky, SS
背景与目的：本研究旨在比较23例成人牙周炎患者(平均年龄51±14岁)上、下菌斑的微生物组成。方法：采用全基因组探针和棋盘式DNA-DNA杂交法，对每例牙近中牙面的1.170个菌斑标本(包括28个上菌斑和28个龈下菌斑)进行细菌分类鉴定。临床评估包括牙龈发红、探诊出血、菌斑堆积和化脓的两种测量方法，以及袋子深度和附着水平的重复测量。每颗牙6个部位。分别计算每个受试者的菌斑计数(水平)、DNA探针计数(百分比)和菌斑定植部位百分比(患病率)。结果：40种菌斑均检出，放线菌在两种菌斑中均有分布，放线菌是两种菌斑中最常见的类群。75%到100%的以上和62%到100%的龈下部位被这5种放线菌中的至少一种定植。与从相同牙面采集的龈下样本中的平均计数相比，龈上样本中的奈氏放线菌基因种1、以色列放线菌、齿状放线菌、粘膜奈瑟氏菌、戈登链球菌、黄色微孢子虫和Sputigena的平均计数显著增加。龈下菌斑样本中黑色普氏杆菌、中间普氏杆菌、福赛氏拟杆菌和牙龈卟啉单胞菌的数量显著增加。龈下标本中“红色”和“橙色”复合体的比例明显较高，而龈上菌斑中“绿色”和“紫色”复合体以及放线菌的比例较高。在相同菌种阴性的牙周上菌斑中可以检测到可疑的牙周病原体。结论：数据表明，牙周上菌斑中可能含有可能的牙周病原体，提示该环境可能是这类菌种传播或再感染的场所。
Background, aims: The purpose of the present study was to compare and relate the microbial composition of supra and subgingival plaque in 23 adult periodontitis subjects (mean age 51+/-14 years).Methods: A total of 1.170 samples of supra and subgingival plaque were collected from the mesial aspect of every tooth (up to 28 supra and 28 subgingival samples) from each subject and evaluated for the presence and levels of 40 bacterial taxa using whole genomic DNA probes and checkerboard DNA-DNA hybridization. Clinical assessments including dichotomous measures of gingival redness, bleeding on probing, plaque accumulation and suppuration, as well as duplicate measures of pocket depth and attachment level. were made at 6 sites per tooth. The counts (levels), % DNA probe count (proportion) and % of sites colonized (prevalence) of each species in supra and separately in subgingival plaque were computed for each subject. Significance of differences between supra and subgingival plaque for each species was sought using the Wilcoxon signed ranks test and adjusted for multiple comparisons.Results: All 40 taxa were detected in both supra and subgingival plaque, Actinomyces species were the most prevalent taxa in both habitats. 75 to 100% of supra and 62 to 100% of subgingival sites were colonized by at least one of the 5 Actinomyces species. Supragingival samples exhibited significantly higher counts of Actinomyces naeslundii genospecies 1, Actinomyces israelii, Actinomyces odontolyticus, Neisseria mucosa, Streptococcus gordonii, Capnocytophaga ochracea and Capnocytophaga sputigena when compared with mean counts in subgingival samples taken from the same tooth surfaces. Subgingival plaque samples presented significantly higher counts of Prevotella nigrescens, Prevotella intermedia, Bacteroides forsythus and Porphyromonas gingivalis. Subgingival samples exhibited a significantly higher proportion of "red" and "orange complex" species, while supragingival plaque exhibited higher proportions of "green" and "purple" complex species as well as Actinomyces species. Suspected periodontal pathogens could be detected in supragingival plaque From sites where subgingival samples were negative for the same species.Conclusions: The data indicate that supragingival plaque can harbor putative periodontal pathogens, suggesting a possible role of this environment as a reservoir of such species for the spread or reinfection of subgingival sites.