CENTROMERIC SATELLITE DNA IN THE NEWT TRITURUS-CRISTATUS-KARELINII AND RELATED SPECIES - ITS DISTRIBUTION AND TRANSCRIPTION ON LAMPBRUSH CHROMOSOMES
CENTROMERIC SATELLITE DNA IN THE NEWT TRITURUS-CRISTATUS-KARELINII AND RELATED SPECIES - ITS DISTRIBUTION AND TRANSCRIPTION ON LAMPBRUSH CHROMOSOMES
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DOI：
10.1007/bf00328461
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发表时间：
1985-01-01
期刊：
影响因子：
1.6
通讯作者：
MACGREGOR, HC
中科院分区：
文献类型：
--
作者：
BALDWIN, L;MACGREGOR, HC
从T. C.卡累利阿。其中较小的具有33个碱基对（bp）的重复单元的被指定为TkS 1，较大的具有68 bp的被指定为TkS 2。这些卫星也存在于T. c的DNA中。cristatus，T. C. carnifex和T. marmoratus，但拷贝数明显较低。用原位杂交技术对T. C. karelinii和T. C. cristatus显示随体集中在T. C. karelinii和T. C.鸡冠。这些随体还特异性地结合到有丝分裂中期染色体的着丝粒区域。它们不与1号染色体的异形臂结合，而1号染色体先前被证明富含高度重复的DNA。与TkS 1的灯刷染色体的DNA/RNA转录物原位杂交表明，该序列偶尔在着丝粒附近的灯刷环上转录。
Two abundant satellite DNA sequences were identified in and cloned from the DNA of T. c. karelinii. The smaller of these with a repeat unit of 33 base pairs (bp) is designated TkS1, the larger with 68 bp is designated TkS2. These satellites are also present in DNA from T.c. cristatus, T. c. carnifex and T. marmoratus but in substantially lower copy number. In situ hybridization to lampbrush chromosomes of T. c. karelinii and T. c. cristatus shows the satellites are concentrated in the heterochromatic centromere bars of T. c. karelinii and in a region around the centromere granule in T. c. cristatus. The satellites also bind specifically to the centromere regions of mitotic metaphase chromosomes. They do not bind to the heteromorphic arms of chromosome 1, which were previously shown to be rich in highly repeated DNA. DNA/RNA-transcript in situ hybrids to lampbrush chromosomes with TkS1 suggest that this sequence is occasionally transcribed on lampbrush loops near the centromeres.