Profiling the neutralizing antibody response in chronically HIV-1 CRF07_BC-infected intravenous drug users naïve to antiretroviral therapy.
Profiling the neutralizing antibody response in chronically HIV-1 CRF07_BC-infected intravenous drug users naïve to antiretroviral therapy.
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DOI：
10.1038/srep46308
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发表时间：
2017-04-07
影响因子：
4.6
通讯作者：
Shao Y
中科院分区：
文献类型：
--
作者：
Hu X;Hu Y;Zhao C;Gao H;Greene KM;Ren L;Ma L;Ruan Y;Sarzotti-Kelsoe M;Montefiori DC;Hong K;Shao Y
表征不同HIV-1毒株感染者的中和抗体（NAb）反应对于揭示区域预防和治疗策略发展的新靶点是必要的。我们使用30种2-3级Env-假型病毒的大型多亚型组评估了98名CRF 07_BC感染个体中NAb应答的流行率、广度和效力。此外，我们比较了中国CRF 07_BC感染者与B '亚型感染者的中和模式。在检测的98份血浆样本中，18%中和了样本组中超过80%的病毒，53%中和了超过50%的病毒，表明这些个体中存在广泛的NAb。CRF 07_BC感染者对亚型内病毒的中和滴度明显高于感染时间较长的B '亚型感染者。然而，亚型B '感染的个体比CRF07_BC感染的个体对亚型间病毒产生更广泛的中和应答，感染时间更短，表明随着时间的推移从狭义自体中和转变为广义异源中和。来自每个队列的前6个血浆的中和活性归因于IgG组分，其中一半产生了CD 4结合位点抗体反应性。热图分析确定了三个统计学上稳健的血浆簇，为进一步深入的病毒学和免疫学研究提供了宝贵的资源。
Characterizing neutralizing antibody (NAb) responses in individuals infected with diverse HIV-1 strains is necessary to reveal the novel targets for regional preventive and therapeutic strategies development. We evaluated the prevalence, breadth, and potency of NAb responses in 98 CRF07_BC-infected individuals using a large, multi-subtype panel of 30 tier 2-3 Env-pseudotyped viruses. Furthermore, we compared the neutralization pattern of CRF07_BC-infected people with that of subtype B’-infected individuals in China. Of the 98 plasma samples tested, 18% neutralized more than 80% of viruses in the panel, and 53% neutralized more than 50%, suggesting the presence of broadly NAbs in these individuals. A preferential intra-subtype neutralization of CRF07_BC was found. Notably, CRF07_BC-infected individuals generated higher neutralization titers against intra-subtype viruses than subtype B’-infected individuals with longer infection length. However, subtype B’-infected individuals mounted broader neutralization responses against inter-subtype viruses than CRF07_BC infection with shorter infection time, indicating the transition from narrow autologous to broad heterologous neutralization over time. Neutralization activity of the top six plasmas from each cohort was attributable to IgG fraction, and half of them developed CD4 binding site antibody reactivity. Heatmap analysis identified three statistically robust clusters of plasmas that offer valuable resources for further in-depth virological and immunological study.
影响因子：
3.3
作者：
Chen, Jianping;Hong, Kunxue;Jia, Mingming;Liu, Hongwei;Zhang, Yuanzhi;Liu, Sha;Zhang, Xiaoqing;Zhao, Hongjing;Peng, Hong;Ma, Pengfei;Xing, Hui;Ruan, Yuhua;Williams, Katie L.;Yu, Xu G.;Altfeld, Marcus;Walker, Bruce D.;Shao, Yiming
通讯作者：
Shao, Yiming