A mean shift algorithm for drift correction in localization microscopy.
A mean shift algorithm for drift correction in localization microscopy.
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DOI：
10.1016/j.bpr.2021.100008
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发表时间：
2021-09-08
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影响因子：
--
通讯作者：
Veatch, Sarah L
中科院分区：
文献类型：
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作者：
Fazekas, Frank J;Shaw, Thomas R;Veatch, Sarah L
Single-molecule localization microscopy techniques transcend the diffraction limit of visible light by localizing isolated emitters sampled stochastically. This time-lapse imaging necessitates long acquisition times, over which sample drift can become large relative to the localization precision. Here, we present an efficient and robust method for estimating drift, using a simple peak-finding algorithm based on mean shifts that is effective for single-molecule localization microscopy in two or three dimensions.
