Poikilolaimus floridensis n. sp (Rhabditida: Rhabditidae) associated with termites (Kalotermitidae)
Poikilolaimus floridensis n. sp (Rhabditida: Rhabditidae) associated with termites (Kalotermitidae)
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DOI：
10.1163/156854109x429547
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发表时间：
2009-01-01
期刊：
影响因子：
1.2
通讯作者：
Center, Barbara J.
中科院分区：
文献类型：
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作者：
Kanzaki, Natsumi;Giblin-Davis, Robin M.;Center, Barbara J.
在佛罗里达南部进行了一项白蚁相关线虫的调查，以比较新热带地区与其他纬度的这种关联的多样性。从田间采集了6种（15个菌落）白蚁（等翅目），从实验室种群中采集了8种（15个菌落）白蚁进行了线虫相关检查。野外采集的6种白蚁，即洞额隐白蚁（Cryptotermes cavifrons）、斯氏巢白蚁（Incisitermes snyderi）、乔氏新白蚁（Neotermes jouteli）、新白蚁（N.栗白蚁（Castaneus）、单纯原鼻白蚁（Prorhinotermes simplex）和黄足散白蚁（Reticulitermes flavipes）代表两个科（Kalotermitidae），后两个物种为鼻白蚁科（Rhinotermitidae）），与线虫有关。线虫也分离自实验室种群的I。snyderi和台湾乳白蚁Coptotermes formosanus。总之，使用分子条形码和培养（当成功时）识别出七种推定的线虫物种，包括四种小杆线虫、一种双腹小杆线虫、雷氏小杆线虫和我们在本文中描述为Poikilolaimus flororescsis n.该线虫是从日本血吸虫N. jouteli，新单胞菌N. Castaneus和I. snyderi。从工蚁、一名士兵和一只有翅虫身上找到的，表明是体内寄生虫。其特征是气孔口有6个三角形表皮片，气孔结构简单，无齿和声门器，分泌排泄孔角质化，无触角，雄尾圆锥形，无囊或刺，雌尾短而圆锥形，雌性生殖系统双形。佛罗里达花鳗sp.是形态特征，不容易符合目前的属定义Poikilolaimus重新定义。基于近全长SSU核糖体DNA序列的分子系统发育分析表明，该新种在该属中处于基础位置。
A survey of termite-associated nematodes was done in southern Florida to compare the diversity of such associations with other latitudes in the Neotropics. Six species (15 colonies) of termites (Isoptera) were collected from the field and eight species (15 colonies) from laboratory populations were examined for nematode associates. All six field-collected termite species, Cryptotermes cavifrons, Incisitermes snyderi, Neotermes jouteli, N. castaneus, Prorhinotermes simplex and Reticulitermes flavipes, representing two families (Kalotermitidae and, for the latter two species, Rhinotermitidae), were associated with nematodes. Nematodes were also isolated from laboratory populations of I. snyderi and Coptotermes formosanus. In total, seven putative species of nematodes were discerned using molecular bar-coding and culturing (when successful) including four rhabditids, one diplogastrid, Rhabditis rainai and a nematode that we are describing herein as Poikilolaimus floridensis n. sp. This nematode was isolated as dauer juveniles in the foregut of N. jouteli, N. castaneus and I. snyderi. It was recovered from workers, a soldier and an alate, suggesting internal phoresy. It is characterised by six triangular cuticular flaps covering the stomatal opening, simple tube-like stomatal structure, i.e., absence of teeth and glottoid apparatus, cuticularised and refractile secretory-excretory pore, conical male tail lacking 'bursa' or spike, short and conical female tail and didelphic female reproductive system. Poikilolaimus floridensis n. sp. is morphologically characteristic and does not easily fit the current genus definition of Poikilolaimus which is redefined herein. Molecular phylogenetic analysis based on near full length SSU ribosomal DNA sequence showed that the new species occupies a basal position in the genus.