Heightened induction of c-jun mRNA by PMA, EGF and IL-1 in ataxia telangiectasia lymphoblasts.
Heightened induction of c-jun mRNA by PMA, EGF and IL-1 in ataxia telangiectasia lymphoblasts.
复制标题
PMA、EGF 和 IL-1 在共济失调毛细血管扩张淋巴母细胞中增强 c-jun mRNA 的诱导。
DOI：
10.1080/095530099139953
复制
发表时间：
1999
影响因子：
2.6
通讯作者：
Mallya,SM
中科院分区：
文献类型：
--
作者：
Sikpi,MO;Wang,Y;Mallya,SM
PurposeTo determine if cells from the cancer-prone autosomal recessive disorder ataxia telangiectasia (A-T) are defective in responding to stimuli other than ionizing-radiation (IR) damage.Materials and methodsThe induction of c-jun transcripts by IR, by phorbol 12-myristate 13-acetate (PMA), interleukin 1 (IL-1) and epidermal growth factor (EGF) in normal and A-T lymphoblasts was measured.ResultsTreatment with PMA increased c-jun transcripts in a dose- and time-dependent manner two- to three-fold more in A-T than in normal cells. Similarly, treatment with EGF and IL-1 also increased c-jun transcripts more in A-T than in normal lymphoblasts. In contrast, exposure to gamma -radiation increased c-jun transcripts at least twofold more in normal than in A-T lymphoblasts.ConclusionsThese findings indicate that A-T cells are not only defective in responding to IR damage, but also in responding to mitogenic stimuli like IL-1 and EGF. Furthermore, these findings implicate ATM, the gene responsible for the A-T disorder, in the induction of c-jun transcripts by PMA, EGF or IL-1.
