Cytochemical studies on the problem of macronuclear subnuclei in tetrahymena.

Cytochemical studies on the problem of macronuclear subnuclei in tetrahymena.
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DOI:
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发表时间:
1972-02
期刊:
影响因子:
3.3
通讯作者:
J. Woodard;E. Kaneshiro;M. Gorovsky
J. Woodard;E. Kaneshiro;M. Gorovsky
中科院分区:
生物学2区
文献类型:
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作者:
J. Woodard;E. Kaneshiro;M. Gorovsky

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用Feulgen显微分光光度法测定了梨形四膜虫大核和微核的DNA含量。假设未复制的微核是二倍体,发现未复制的大核含有约45倍的单倍体DNA量。讨论了这些发现与艾伦和南尼及其合作者表征的45个独立分类遗传亚基的关系。还描述了细胞周期中大核和微核的DNA合成模式。
DNA amounts in macronuclei and micronuclei of Tetrahymena pyriformis were measured by Feulgen microspectrophotometry. Assuming that the unreplicated micronucleus is diploid, the unreplicated macronucleus was found to contain approximately 45 times the haploid DNA amount. The relationship of these findings to the 45 independently assorting genetic subunits characterized by Allen and Nanney and their collaborators is discussed. The pattern of DNA synthesis in macro- and micronuclei during the cell cycle is also described.