Acute Changes in Circulating Concentrations of Progesterone and Nitric Oxide and Partial Pressure of Oxygen During Prostaglandin F2α-induced Luteolysis in Cattle
Acute Changes in Circulating Concentrations of Progesterone and Nitric Oxide and Partial Pressure of Oxygen During Prostaglandin F2α-induced Luteolysis in Cattle
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DOI：
10.1262/jrd.20133
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发表时间：
2009-04-01
影响因子：
1.8
通讯作者：
Skarzynski, Dariusz Jan
中科院分区：
文献类型：
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作者：
Acosta, Tomas Javier;Bah, Mamadou Bousa;Skarzynski, Dariusz Jan
为了检查氧 (O-2) 和一氧化氮 (NO) 是否与黄体溶解期间黄体功能的急性变化存在时间相关性，我们测定了前列腺素 F-2 α (PGF(2 α) 诱导的牛黄体溶解期间黄体酮 (P4) 和亚硝酸盐/硝酸盐（NO 的稳定代谢物）循环浓度以及氧分压 (pO(2)) 的实时变化。在发情周期第 9 天（发情=第 0 天）将样品收集插入 12 头奶牛的卵巢静脉 (OV)、颈静脉 (JV) 和腹主动脉 (AA)。在第 10 天，将奶牛随机分为两组，并用溶黄体剂量的 PGF(2α) 类似物或盐溶液（对照）在 -2、-1、0、 0.25、0.5、0.75、1 和 2 小时，然后每隔 2 小时注射一次 PGF(2 α)，直至治疗后 12 小时（0 小时），注射 PGF(2 α) 会在 2 小时内导致 OV 血浆中 P4 浓度显着降低。比合资血液中的（P
To examine whether oxygen (O-2) and nitric oxide (NO) are temporally associated with the acute changes in luteal function during luteolysis, we determined the real-time changes in the circulating concentrations of progesterone (P4) and nitrite/nitrate (the stable metabolites of NO) and the partial pressure of oxygen (pO(2)) during prostaglandin F-2 alpha (PGF(2 alpha)-induced luteolysis in cattle. Catheters for frequent blood sample collection were inserted into the ovarian vein (OV), jugular vein (JV) and aorta abdominalis (AA) in 12 cows on Day 9 of the oestrous cycle (oestrus=Day 0). On Day 10, the cows were randomly divided into two groups and treated with a luteolytic dose of a PGF(2 alpha) analogue or saline solution (control). Blood samples were collected at -2, -1, 0, 0.25, 0.5, 0.75, 1 and 2 h and then at 2-h intervals until 12 h after treatment (0 h). Injection of a PGF(2 alpha) induced a significant decrease in the concentrations of P4 in OV plasma within 2 h. The decrease in P4 concentrations was preceded by an increase in the NO concentrations in the blood collected from OV, JV and AA. Basal pO(2) was significantly higher in OV blood than in JV blood (P