Cultivar variation and selection potential relevant to the production of cellulosic ethanol from wheat straw
Cultivar variation and selection potential relevant to the production of cellulosic ethanol from wheat straw
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DOI：
10.1016/j.biombioe.2011.12.009
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发表时间：
2012-02-01
影响因子：
6
通讯作者：
Wyman, C. E.
中科院分区：
文献类型：
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作者：
Lindedam, J.;Andersen, S. B.;Wyman, C. E.
优化纤维素乙醇产率强烈依赖于对原料生物学变异的理解。本研究旨在通过高通量预处理和水解孔板技术，研究不同冬小麦品种秸秆发酵产糖能力的差异。这项技术使我们能够估计小麦秸秆栽培品种的糖产量，化学成分，农艺品质和植物部分的分布之间的相关性和环境。在丹麦的两个地点一式两份地收集来自20个栽培品种的秸秆。在水热预处理（180 ℃，17.6分钟）和共水解后，测量糖释放和糖转化率。观察到品种之间的糖释放差异高达26%。糖释放量与木质素和灰分含量呈负相关，与碳水化合物含量和株高呈正相关。纤维素的可获得性会阻碍糖转化率，并且每个植物级分的可转化性可能比植物级分的相对比例对总体糖转化更重要。我们的研究结果表明，选择品种的小麦秸秆生物燃料原料是可能的，因为糖释放的遗传率为57%，有一些其他农艺性状的负相关。(C)2011爱思唯尔有限公司保留所有权利。
Optimizing cellulosic ethanol yield depends strongly on understanding the biological variation of feedstocks. Our objective was to study variation in capacity for producing fermentable sugars from straw of winter wheat cultivars with a high-throughput pretreatment and hydrolysis well-plate technique. This technique enabled us to estimate cultivar-related and environmental correlations between sugar yield, chemical composition, agronomic qualities, and distribution of botanical plant parts of wheat straw cultivars. Straws from 20 cultivars were collected in duplicates on two sites in Denmark. Following hydrothermal pretreatment (180 degrees C for 17.6 min) and co-hydrolysis, sugar release and sugar conversion were measured. Up to 26% difference in sugar release between cultivars was observed. Sugar release showed negative cultivar correlation with lignin and ash content, whereas sugar release showed positive cultivar correlation with content of carbohydrates and plant height. Accessibility to cellulose can impede the sugar conversion rate, and convertibility of each botanical fraction might be more important to overall sugar conversion than the relative proportions of botanical fractions. Our results suggest that selection of cultivars for improved biofuel feedstock of wheat straw is possible, because heritability of sugar release is 57% and there are few adverse correlations to other agronomic traits. (C) 2011 Elsevier Ltd. All rights reserved.