Progress in high throughput SNP genotyping methods
Progress in high throughput SNP genotyping methods
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DOI：
10.1038/sj.tpj.6500094
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发表时间：
2002-01-01
影响因子：
2.8
通讯作者：
Dracopoli, N. C.
中科院分区：
文献类型：
--
作者：
Tsuchihashi, Z.;Dracopoli, N. C.
大多数目前的单核苷酸多态性（SNP）基因分型方法仍然太慢和昂贵的常规使用在大量的DNA样本中的数百个或更多的SNP的大型关联研究。然而，SNP基因分型技术正在迅速发展，新的，更快，更便宜的方法的出现以及现有方法的改进。在这篇综述中，我们重点介绍了旨在高通量使用的技术，并讨论了过去几年在这一领域取得的技术进展。技术的快速进步，结合数百万SNP的发现和人类单倍型图谱的开发，可能使全基因组关联研究在不久的将来启动。
Most current single nucleotide polymorphism (SNP) genotyping methods are still too slow and expensive for routine use in large association studies with hundreds or more SNPs in a large number of DNA samples. However, SNP genotyping technology is rapidly progressing with the emergence of novel, faster and cheaper methods as well as improvements in the existing methods. In this review, we focus on technologies aimed at high throughput uses, and discuss the technical advances made in this field in the last few years. The rapid progress in technology, in combination with the discovery of millions of SNPs and the development of the human haplotype map, may enable whole genome association studies to be initiated in the near future.