Phospholipase C activity in plasma membranes isolated from lapine synovial cells in monolayer culture.
Phospholipase C activity in plasma membranes isolated from lapine synovial cells in monolayer culture.
复制标题
从单层培养的兔滑膜细胞中分离出的质膜中的磷脂酶 C 活性。
DOI：
10.1016/0006-291x(83)91008-2
复制
发表时间：
1983
影响因子：
3.1
通讯作者：
Malemud,CJ
中科院分区：
文献类型：
--
作者：
Rothenberg,RJ;Moskowitz,RW;Malemud,CJ
使用不连续蔗糖梯度离心技术从单层培养中生长的兔滑膜细胞中分离质膜。检测到大量的 5' 核苷酸酶，而 6 磷酸葡萄糖脱氢酶和细胞色素氧化酶的含量很低，甚至无法检测到。将质膜与[3H]-花生四烯酰-磷脂酰肌醇/磷脂酰丝氨酸在37℃下孵育60分钟，合成[3H]-二酰基甘油酯。当使用[3H]-花生四烯酰-磷脂酰胆碱或[3H]-花生四烯酰-磷脂酰乙醇胺作为底物时，几乎没有测量到[3H]-二酰基甘油酯的合成。这些结果与来自培养的兔子滑膜细胞的质膜相关磷脂酰肌醇特异性磷脂酶 C 一致。
Plasma membranes were isolated from lapine synovial cells grown in monolayer culture using discontinuous sucrose gradient centrifugation techniques. 5′nucleotidase was detected in great abundance while glucose-6 phosphate dehydrogenase and cytochrome oxidase were present at low to undetectable levels. Plasma membranes incubated at 37°C for 60 min with [3H]-arachidonyl-phosphatidylinositol/phosphatidylserine synthesized [3H]-diacylglycerides. Little if any [3H]-diacylglyceride synthesis was measured when [3H]-arachidonyl-phosphatidylcholine or [3H]-arachidonyl-phosphatidylethanolamine were used as substrates. These results are consistent with a plasma membrane-associated phosphatidylinositol-specific phospholipase C from lapine synovial cells in culture.