Metabolic profile biomarkers of metal contamination in a sentinel terrestrial species are applicable across multiple sites
Metabolic profile biomarkers of metal contamination in a sentinel terrestrial species are applicable across multiple sites
复制标题
DOI：
10.1021/es0700303
复制
发表时间：
2007-06-15
影响因子：
11.4
通讯作者：
Morgan, A. John
中科院分区：
文献类型：
--
作者：
Bundy, Jacob G.;Keun, Hector C.;Morgan, A. John
在这项研究中，我们解决了一个问题，是否组学方法可以真正有用的生物标志物分布分析在不同的领域网站具有不同的理化特性。我们收集了蚯蚓（红蚯蚓）从7个网站有非常不同的金属污染水平和流行的土壤类型和H-1核磁共振光谱分析组织提取物。模式识别分析的数据表明，这两个网站和污染物的具体影响的代谢概况可以辨别。锌被确定为引起蚯蚓代谢变化的可能主要污染物。个别网站可以通过主成分分析的基础上解决的NMR光谱配置文件，这些网站的差异也可能是由额外的非生物因素，如土壤pH值。尽管不可避免的程度混淆网站和污染物的浓度，它是可能的，以确定与锌在所有不同的网站的代谢产物。因此，这项研究作为一个基于NMR的代谢谱作为诊断工具，在污染的农田土壤生态毒理学研究的原则证明。
In this study, we addressed the question of whether an omic approach could genuinely be useful for biomarker profile analysis across different field sites with different physicochemical characteristics. We collected earthworms (Lumbricus rubellus) from seven sites with very different levels of metal contamination and prevailing soil type and analyzed tissue extracts by H-1 nuclear magnetic resonance spectroscopy. Pattern recognition analysis of the data showed that both site- and contaminant-specific effects on the metabolic profiles could be discerned. Zinc was identified as the probable major contaminant causing a metabolic change in the earthworms. Individual sites could be resolved on the basis of NMR spectral profiles by principal component analysis; these site differences may also have been caused by additional abiotic factors such as soil pH. Despite an inevitable degree of confounding between site and contaminant concentrations, it was possible to identify metabolites which were correlated with zinc across all different sites. This study therefore acts as a proof of principle for the use of NMR-based metabolic profiling as a diagnostic tool for ecotoxicological research in polluted field soils.