Quantitative phase imaging with molecular vibrational sensitivity
Quantitative phase imaging with molecular vibrational sensitivity
复制标题
DOI：
10.1364/ol.44.003729
复制
发表时间：
2019-08-01
期刊：
影响因子：
3.6
通讯作者：
Ideguchi, Takuro
中科院分区：
文献类型：
--
作者：
Tamamitsu, Miu;Toda, Keiichiro;Ideguchi, Takuro
定量相位成像 (QPI) 可量化样本特定的光学相位延迟，从而能够对生物样本等光学透明样本进行客观研究，但缺乏化学敏感性，限制了其在基于形态的诊断中的应用。我们提出了利用中红外 (MIR) 光热效应在 QPI 框架中实现的宽视场分子振动 (MV) 显微镜。我们的技术在 1450 - 1640 cm(-1) 的分子指纹区域提供与传统红外光谱仪相当的 MIR 光谱性能，并以每秒 1 帧的速度在 100 μ m x 100 μm 区域上实现二氧化硅-聚苯乙烯珠混合物的宽场分子成像，空间分辨率为 430 nm，与相比，与 10 pJ/μ m(2) 相比，通量降低了 2-3 个数量级，类似于 10 pJ/μ m(2)其他高速无标记分子成像方法，减少对样品的光损伤。借助高能中红外脉冲源，我们的技术可以实现定量形态和MV对比度的高速、无标记、同步、原位采集，为光学透明复杂动力学的研究提供新的见解。 (c) 2019 年美国光学学会
Quantitative phase imaging (QPI) quantifies the sample-specific optical-phase-delay enabling objective studies of optically transparent specimens such as biological samples but lacks chemical sensitivity, limiting its application to a morphology-based diagnosis. We present wide-field molecular vibrational (MV) microscopy realized in the framework of QPI utilizing a mid-infrared (MIR) photothermal effect. Our technique provides MIR spectroscopic performance comparable to that of a conventional infrared spectrometer in the molecular fingerprint region of 1450 - 1640 cm(-1) and realizes wide-field molecular imaging of a silica-polystyrene bead mixture over a 100 mu m x 100 mu m area at 1 frame per second with the spatial resolution of 430 nm and 2-3 orders of magnitude lower fluence of similar to 10 pJ/mu m(2) compared to other high-speed label-free molecular imaging methods, reducing photodamages to the sample. With a high-energy MIR pulse source, our technique could enable high-speed, label-free, simultaneous, and in situ acquisition of quantitative morphology and MV contrast, providing new insights for studies of optically transparent complex dynamics. (c) 2019 Optical Society of America