Effect of dietary fish oil intake on ubiquitin ligase expression during muscle atrophy induced by sciatic nerve denervation in mice
Effect of dietary fish oil intake on ubiquitin ligase expression during muscle atrophy induced by sciatic nerve denervation in mice
复制标题
膳食鱼油摄入量对坐骨神经去神经引起的小鼠肌肉萎缩期间泛素连接酶表达的影响
DOI：
10.1111/asj.13224
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发表时间：
2019
影响因子：
2
通讯作者：
Arihara K
中科院分区：
文献类型：
--
作者：
Komiya Y;Kobayashi C;Uchida N;Otsu S;Tanio T;Yokoyama I;Nagasao J;Arihara K
在几种肌肉萎缩模型中，饮食中摄入鱼油可以改善肌肉萎缩，但对去神经支配诱导的肌肉萎缩的影响尚不清楚。因此，本研究旨在探讨鱼油对小鼠坐骨神经去神经诱导的肌肉萎缩及肌肉萎缩标志物表达的影响。我们对鱼油饲喂坐骨神经去神经小鼠肌肉萎缩标志物进行了组织学和定量mRNA表达分析。组织学分析表明，食用鱼油对去神经治疗引起的肌纤维直径减少有轻微的抑制作用。此外，膳食鱼油摄入抑制了去神经支配治疗上调的MuRF1（含三方基元63）表达，这是由于骨骼肌中肿瘤坏死因子α (TNF - α)的产生减少。我们得出结论，在小鼠坐骨神经去神经支配引起的肌肉萎缩过程中，饮食中摄入鱼油通过降低TNF - α的产生来抑制MuRF1的表达。
Dietary fish oil intake improves muscle atrophy in several atrophy models however the effect on denervation‐induced muscle atrophy is not clear. Thus, the aim of this study was to investigate the effects of dietary fish oil intake on muscle atrophy and the expression of muscle atrophy markers induced by sciatic nerve denervation in mice. We performed histological and quantitative mRNA expression analysis of muscle atrophy markers in mice fed with fish oil with sciatic nerve denervation. Histological analysis indicated that dietary fish oil intake slightly prevented the decrease of muscle fiber diameter induced by denervation treatment. In addition, dietary fish oil intake suppressed the MuRF1 (tripartite motif‐containing 63) expression up‐regulated by denervation treatment, and this was due to decreased tumor necrosis factor‐alpha (TNF‐α) production in skeletal muscle. We concluded that dietary fish oil intake suppressed MuRF1 expression by decreasing TNF‐α production during muscle atrophy induced by sciatic nerve denervation in mice.