A metabonomic approach to early prognostic evaluation of experimental sepsis by 1H NMR and pattern recognition
A metabonomic approach to early prognostic evaluation of experimental sepsis by 1H NMR and pattern recognition
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DOI：
10.1002/nbm.1373
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发表时间：
2009-07-01
影响因子：
2.9
通讯作者：
Zhang, Wei-dong
中科院分区：
文献类型：
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作者：
Lin, Zhong-ying;Xu, Ping-bo;Zhang, Wei-dong
本研究提出了一种基于核磁共振的代谢组学方法来评估脓毒症的早期预后。40只接受盲肠结扎穿孔术（CLP）的脓毒症大鼠于第6天分为存活组和死亡组，20只假手术大鼠作为对照组。在手术后12小时从脓毒症和假手术大鼠中收集血清样品，并使用H-1 NMR光谱进行分析。应用正交偏最小二乘法（OPLS），并根据预定义的组显示聚类，表明基于NMR的代谢分析可以揭示假手术，存活和非存活脓毒症大鼠血清中的病理特征。此外，脓毒症大鼠体内主要参与能量代谢的6种特征性代谢产物乳酸、丙氨酸、乙酸、乙酰乙酸、羟基丁酸和甲酸发生了显著变化，尤其是在死亡大鼠体内。使用这些代谢物，预测模型的预后评估脓毒症，使用径向基函数神经网络（RBFNN）的预测精度约为87%的测试样品构建。结果表明，基于NMR的代谢组学方法是一种潜在的技术，脓毒症的早期预后评估。版权所有（C）2009约翰威利父子有限公司
This study proposes an NMR-based metabonomic approach to early prognostic evaluation of sepsis. Forty septic rats receiving cecal ligation and puncture (CLP) were divided into the surviving group and nonsurviving group on day 6, while 20 sham-operated rats served as the control group. Serum samples were collected from septic and sham-operated rats at 12 h after surgery and analyzed using H-1 NMR spectroscopy. Orthogonal partial least squares (OPLS) were applied and showed clustering according to predefined groups, indicating that NMR-based metabolic profiling could reveal pathologic characteristics in the serum of sham-operated, surviving, and nonsurviving septic rats. In addition, six characteristic metabolites including lactate, alanine, acetate, acetoacetate, hydroxybutyrate, and formate, which are mainly involved in energy metabolism, changed markedly in septic rats, especially in the nonsurvivors. Using these metabolites, a predictive model for prognostic evaluation of sepsis was constructed using a radial basis function neural network (RBFNN) with a prediction accuracy of about 87% by test samples. The results indicated that the NMR-based metabonomic approach is a potential technique for the early prognostic evaluation of sepsis. Copyright (C) 2009 John Wiley & Sons, Ltd.