Neuropeptide Y in the dorsal vagal complex stimulates bicarbonate-dependent bile secretion in rats.
Neuropeptide Y in the dorsal vagal complex stimulates bicarbonate-dependent bile secretion in rats.
复制标题
背侧迷走神经复合体中的神经肽 Y 刺激大鼠碳酸氢盐依赖性胆汁分泌。
DOI：
10.1016/s0016-5085(97)70050-7
复制
发表时间：
1997
期刊：
影响因子：
29.4
通讯作者：
I. Makino
中科院分区：
文献类型：
--
作者：
M. Yoneda;S. Yokohama;K. Tamori;Y. Sato;K. Nakamura;I. Makino
背景与目的：在动物模型中，中枢给药神经肽Y （NPY）可通过迷走神经通路增强胆汁分泌。NPY神经纤维和受体定位于迷走背复合体（DVC），逆行示踪技术显示肝迷走神经主要从左侧DVC投射。然而，对NPY诱导胆汁分泌的中枢作用位点尚不清楚。本研究对NPY作用的髓质部位进行了研究。方法：采用氨氨麻醉胆汁酸代偿大鼠胆管插管。测量基础分泌量后，将NPY微注射到DVC中，观察100分钟的胆汁反应。肽前2小时行左、右颈迷走神经切断术或肝支迷走神经切断术。结果：左DVC微量注射NPY (7- 30pmol)，但右DVC不注射，剂量依赖性地增加了胆汁酸依赖型和碳酸氢盐依赖型胆汁分泌。左颈和肝支迷走神经切开术可消除NPY对胆汁分泌的刺激，但右颈迷走神经切开术不能消除NPY对胆汁分泌的刺激。结论：NPY作用于左侧DVC，通过左侧颈迷走神经和肝迷走神经刺激胆汁酸非依赖性和碳酸氢盐依赖性胆汁分泌；这些发现提示神经肽可能在特定的脑核中起调节肝功能的作用。（Gastroenterology 1997; 5;112(5):1673-80）
BACKGROUND & AIMS:Central administration of neuropeptide Y (NPY) enhances bile secretion through vagal pathways in animal models. NPY nerve fibers and receptors are localized in the dorsal vagal complex (DVC), and retrograde tracing techniques have shown that hepatic vagal nerves are projected mainly from the left DVC. However, nothing is known about the central sites of action for NPY to elicit bile secretion. The medullary sites of the action for NPY were investigated in this study.METHODS:The bile duct was cannulated in urethane- anesthetized and bile acid-compensated rats. After measuring basal secretion, NPY was microinjected into the DVC and bile response was observed for 100 minutes. Either left or right cervical vagotomy or hepatic branch vagotomy was performed 2 hours before the peptide.RESULTS:Microinjection of NPY (7-30 pmol) into the left DVC, but not the right DVC, dose-dependently increased bile acid-independent and bicarbonate-dependent bile secretion. Stimulation of bile secretion by NPY was eliminated by left cervical and hepatic branch vagotomy but not by right cervical vagotomy.CONCLUSIONS:NPY acts in the left DVC to stimulate bile acid-independent and bicarbonate-dependent bile secretion through the left cervical and hepatic vagus; these findings suggest that neuropeptides may act in the specific brain nuclei to regulate hepatic function. (Gastroenterology 1997 May;112(5):1673-80)