Glycoprofiling Bifidobacterial Consumption of Galacto-Oligosaccharides by Mass Spectrometry Reveals Strain-Specific, Preferential Consumption of Glycans
Glycoprofiling Bifidobacterial Consumption of Galacto-Oligosaccharides by Mass Spectrometry Reveals Strain-Specific, Preferential Consumption of Glycans
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DOI：
10.1128/aem.00842-09
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发表时间：
2009-12-01
影响因子：
4.4
通讯作者：
Lebrilla, Carlito B.
中科院分区：
文献类型：
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作者：
Barboza, Mariana;Sela, David A.;Lebrilla, Carlito B.
低聚半乳糖 (GOS) 是具有益生元特性的多功能食品成分。然而，目前缺乏精确的分析方法来证明双歧杆菌特定的GOS消耗量。为了更好地了解GOS作为益生元的作用，制备了不含二糖和单糖的纯化GOS（pGOS）并用于细菌发酵实验。生长曲线显示所有检测的双歧杆菌均在 pGOS 制剂上利用并生长。我们使用一种新颖的质谱方法，包括基质辅助激光解吸电离-傅里叶变换离子回旋共振 (MALDI-FTICR) 来确定 GOS 糖浆制剂中低聚糖的组成。对用过的发酵培养基的 MALDI-FTICR 分析表明，不同的双歧杆菌优先消耗选定的 pGOS 物种。这里描述的方法表明 MALDI-FTICR 是一种快速通量工具，用于对 GOS 混合物中的寡糖进行全面分析。此外，不同双歧杆菌对某些 GOS 物种的选择性消耗表明了一种针对益生元来富集选定双歧杆菌物种的方法。
Galacto-oligosaccharides (GOS) are versatile food ingredients that possess prebiotic properties. However, at present there is a lack of precise analytical methods to demonstrate specific GOS consumption by bifidobacteria. To better understand the role of GOS as prebiotics, purified GOS (pGOS) without disaccharides and monosaccharides was prepared and used in bacterial fermentation experiments. Growth curves showed that all bifidobacteria assayed utilized and grew on pGOS preparations. We used a novel mass spectrometry approach involving matrix-assisted laser desorption ionization-Fourier transform ion cyclotron resonance (MALDI-FTICR) to determine the composition of oligosaccharides in GOS syrup preparations. MALDI-FTICR analysis of spent fermentation media demonstrated that there was preferential consumption of selected pGOS species by different bifidobacteria. The approach described here demonstrates that MALDI-FTICR is a rapid-throughput tool for comprehensive profiling of oligosaccharides in GOS mixtures. In addition, the selective consumption of certain GOS species by different bifidobacteria suggests a means for targeting prebiotics to enrich select bifidobacterial species.