ADRENERGIC-INNERVATION AND NEUROGENIC RESPONSE IN LARGE AND SMALL ARTERIES AND VEINS FROM THE RAT
ADRENERGIC-INNERVATION AND NEUROGENIC RESPONSE IN LARGE AND SMALL ARTERIES AND VEINS FROM THE RAT
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DOI：
10.1111/j.1748-1716.1986.tb07795.x
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发表时间：
1986-01-01
期刊：
影响因子：
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通讯作者：
NILSSON, O
中科院分区：
文献类型：
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作者：
NILSSON, H;GOLDSTEIN, M;NILSSON, O
对大鼠内脏脉管系统中肾上腺素能血管收缩神经支配的特性进行了形态学和生理学的联合分析。研究了三代血管：（1）腹主动脉和腔静脉； (2)肠系膜上动、静脉； (3)肠系膜上动脉的200μm分支及其平行静脉。通过 Hillarp-Falck 荧光技术以及酪氨酸羟化酶和多巴胺-β-羟化酶免疫反应的免疫组织化学定位，使肾上腺素能神经支配可视化。在安装在肌动记录仪中的环形制剂上测定对应用的去甲肾上腺素、透壁神经刺激和肌肉直接激活的反应。发现所有血管的外膜内侧边界均受到不同密度的去甲肾上腺素能神经的支配；小动脉的神经密度最高，腹主动脉的神经支配最少。当与应用去甲肾上腺素的最大等长反应相关时，最大神经源性反应与肾上腺素能神经支配的密度平行。然而，血管间半最大反应的频率变化不能仅归因于神经支配特性。等渗条件下，主动脉的收缩量为初始周长的 20%，小动脉和静脉的收缩量为初始周长的 30%。对于等长和等张反应，对应用的去甲肾上腺素的反应和对神经刺激的反应之间的关系相似。在较小的静脉中，观察到β受体介导的去甲肾上腺素反应下降。这种下降在神经源性反应中不太明显。结果表明，从近端动脉到外周动脉，神经分布逐渐密集，神经源性反应也逐渐增强。在静脉侧仅发现微小差异。
A combined morphological and physiological analysis of the properties of the adrenergic vasoconstrictor innervation was carried out in the splanchnic vasculature in the rat. Three generations of vessels were studied: (1) the abdominal aorta and the caval vein; (2) the superior mesenteric artery and vein; (3) 200 μm branches of the superior mesenteric artery and their parallelling veins. The adrenergic innervation was visualized by the Hillarp‐Falck fluorescence technique, and by the immunohistochemical localization of immunoreactivity to tyrosine hydroxylase and dopamine‐β‐hydroxylase. Determination of responses to applied noradrenaline, to transmural nerve stimulation and to direct activation of the muscle was made on ring preparations mounted in a myograph. All vessels were found to be innervated at the adventitio‐medial border by noradrenergic nerves with varying density; the small arteries had the highest nerve density, the abdominal aorta was least innervated. When related to the maximal isometric response to applied noradrenaline, the maximal neurogenic response parallelled the density of the adrenergic innervation. Variations in frequency for half‐maximal response among the vessels could not, however, be ascribed to innervation properties only. The constriction under isotonic conditions amounted to 20% of the initial circumference in the aorta, and to 30% in the small arteries and veins. The relation between response to applied noradrenaline and to nerve stimulation was similar for isometric and isotonic responses. In the smaller veins, a β‐receptor‐mediated decline in the response to applied noradrenaline was seen. This decline was much less pronounced in neurogenic responses. The results indicate a gradation from proximal to peripheral arteries towards denser innervation and greater neurogenic responses. On the venous side only minor differences were found.