The effect of aging on migration, proliferation, and collagen expression of tenocytes in response to ciprofloxacin
The effect of aging on migration, proliferation, and collagen expression of tenocytes in response to ciprofloxacin
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DOI：
10.1002/jor.21576
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发表时间：
2012-05-01
影响因子：
2.8
通讯作者：
Yang, Yun-Ming
中科院分区：
文献类型：
--
作者：
Chang, Hsiang-Ning;Pang, Jong-Hwei S.;Yang, Yun-Ming
喹诺酮引起的肌腱病或肌腱断裂往往与年龄有关。然而，喹诺酮和衰老对肌腱细胞的协同作用仍有待探索。用环丙沙星处理两个年龄组（年轻：2个月；近衰老（老年）：24个月）的大鼠跟腱固有的肌腱细胞。分别通过Transwell过滤器迁移测定和MTT（3-[4,5-二甲基噻唑-2-基]-2,5-二苯基溴化四唑）测定评估肌腱细胞迁移和增殖。分别通过逆转录聚合酶链式反应 (RT/PCR) 和蛋白质印迹分析测定 I 型和 III 型胶原蛋白的信使 RNA 和蛋白表达。 Transwell过滤器迁移测定显示，环丙沙星抑制肌腱细胞迁移，这在老肌腱细胞中变得更加显着（p？
Quinolone-induced tendinopathy or tendon rupture tends to be age-related. However, the synergistic effects of quinolone and aging on tenocytes remained to be explored. Tenocytes intrinsic to rat Achilles tendon from two age groups (young: 2 months; and near senescent (old): 24 months) were treated with ciprofloxacin. Tenocyte migration and proliferation were assessed by transwell filter migration assay and MTT (3-[4,5-Dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide) assay, respectively. Messenger RNA and protein expressions of types I and III collagen were determined by reverse transcription-polymerase chain reaction (RT/PCR) and Western blot analysis, respectively. Transwell filter migration assay revealed that ciprofloxacin inhibited tenocytes migration, which became more significant in old tenocytes (p?