Antioxidant and pro-oxidant properties of pyrroloquinoline quinone (PQQ): implications for its function in biological systems
Antioxidant and pro-oxidant properties of pyrroloquinoline quinone (PQQ): implications for its function in biological systems
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DOI：
10.1016/s0006-2952(02)01453-3
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发表时间：
2003-01-01
影响因子：
5.8
通讯作者：
Murphy, MP
中科院分区：
文献类型：
--
作者：
He, K;Nukada, H;Murphy, MP
吡咯喹啉醌（PQQ）是近年来在母乳中发现的一种新型氧化还原辅因子。据报道，它在细胞培养实验和人类疾病的动物模型中作为一种必需营养素、抗氧化剂和氧化还原调节剂发挥作用。由于线粒体对氧化损伤特别敏感，我们研究了PQQ在离体大鼠肝线粒体中的抗氧化特性。PQQ是一种有效的抗氧化剂，保护线粒体免受氧化应激诱导的脂质过氧化，蛋白质羰基形成和线粒体呼吸链失活。相比之下，PQQ引起培养物中细胞的广泛细胞死亡。这种令人惊讶的效果被过氧化氢酶抑制，并且被证明是由于在培养基中PQQ的自氧化过程中产生过氧化氢。我们的结论是，PQQ的反应性是依赖于它的环境，它可以作为一种抗氧化剂或促氧化剂在不同的生物系统。(C)2002年爱思唯尔科技有限公司All rights reserved.
Pyrroloquinoline quitione (PQQ) is a novel redox cofactor recently found in human milk. It has been reported to function as an essential nutrient, antioxidant and redox modulator in cell culture experiments and in animal models of human diseases. As mitochondria are particularly susceptible to oxidative damage we studied the antioxidant properties of PQQ in isolated rat liver mitochondria. PQQ was an effective antioxidant protecting mitochondria against oxidative stress-induced lipid peroxidation, protein carbonyl formation and inactivation of the mitochondrial respiratory chain. In contrast, PQQ caused extensive cell death to cells in culture. This surprising effect was inhibited by catalase, and was shown to be due to the generation of hydrogen peroxide during the autoxidation of PQQ in culture medium. We conclude that the reactivities of PQQ are dependent on its environment and that it can act as an antioxidant or a pro-oxidant in different biological systems. (C) 2002 Elsevier Science Inc. All rights reserved.