Influence of quantum dot's quantum yield to chemiluminescent resonance energy transfer
Influence of quantum dot's quantum yield to chemiluminescent resonance energy transfer
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量子点量子产率对化学发光共振能量转移的影响
DOI：
10.1016/j.aca.2008.01.018
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发表时间：
2008-03-03
影响因子：
6.2
通讯作者：
Zhao, Yuan-Di
中科院分区：
文献类型：
--
作者：
Wang, Hai-Qiao;Li, Yong-Qiang;Zhao, Yuan-Di
研究了化学发光供体（鲁米诺-H2O2 系统）和量子点（QD，发射波长 593 nm）受体 (CRET) 之间的共振能量转移。比较了油溶性QD、水溶性QD（用巯基乙酸盐修饰）和QD-HRP缀合物作为受体时的共振能量转移效率。在这三种情况下都可以观察到QD的荧光，表明在使用不同状态的QD受体时发生了CRET。在油溶性QD的情况下获得最高的CRET效率（10.7％），在QD-HRP缀合物的情况下观察到最低的CRET效率（2.7％）。这个结果与受体的量子产率（18.3％和0.4％）一致。在另一组类似的实验中观察到相同的结果，其中使用了两亲性聚合物修饰的量子点（在 675 nm 处发射）。这表明不同状态下QD的量子产率是CRET效率的关键因素。此外，同时观察到鲁米诺供体和三种不同大小的QD受体之间的多重CRET。该工作将为完善基于量子点的CRET研究提供有益的支持。 (c) 2008 Elsevier B.V. 保留所有权利。
The resonance energy transfer between chemiluminescence donor (luminol-H2O2 system) and quantum dots (QDs, emission at 593 nm) acceptors (CRET) was investigated. The resonance energy transfer efficiencies were compared while the oil soluble QDs, water soluble QDs (modified with thioglycolate) and QD-HRP conjugates were used as acceptor. The fluorescence of QD can be observed in the three cases, indicating that the CRET occurs while QD acceptor in different status was used. The highest CRET efficiency (10.7%) was obtained in the case of oil soluble QDs, and the lowest CRET efficiency (2.7%) was observed in the QD-HRP conjugates case. This result is coincident with the quantum yields of the acceptors (18.3% and 0.4%). The same result was observed in another similar set of experiment, in which the amphiphilic polymer modified QDs (emission at 675 nm) were used. It suggests that the quantum yield of the QD in different status is the crucial factor to the CRET efficiency. Furthermore, the multiplexed CRET between luminol donor and three different sizes QD acceptors was observed simultaneously. This work will offer useful support for improving the CRET studies based on quantum dots. (c) 2008 Elsevier B.V. All rights reserved.