Influence of Two Acyclic Homoterpenes (Tetranorterpenes) on the Foraging Behavior of Anthonomus grandis Boh
Influence of Two Acyclic Homoterpenes (Tetranorterpenes) on the Foraging Behavior of Anthonomus grandis Boh
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DOI：
10.1007/s10886-016-0691-1
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发表时间：
2016-04-01
影响因子：
2.3
通讯作者：
Blassioli-Moraes, Maria Carolina
中科院分区：
文献类型：
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作者：
Magalhes, D. M.;Borges, M.;Blassioli-Moraes, Maria Carolina
以往的研究表明，棉铃象甲，Anthonomus grandis，被吸引到组成和同种植食性昆虫诱导的棉花挥发物，更喜欢混合排放的棉花在生殖阶段的营养阶段。此外，这种偏好被无环高萜（四降萜）（E）-4，8-二甲基-1，3，7-壬三烯（DMNT）和（E，E）-4，8，12-三甲基十三碳-1，3，7，11-四烯（TMTT）在Delta Opal棉花中的营养阶段比生殖阶段更高的释放所证实。在这里，我们评估了这种差异释放的无环高萜类也发生在其他棉花品种，如果棉铃象甲可以识别这些化合物作为一个特定的棉花物候期的指标。结果表明，棉花基因型CNPA TB-90，BRS-293和三角洲蛋白石都产生较高水平的DMNT和TMTT在营养阶段比在生殖阶段，这些高萜类物质允许主成分分析分离的挥发物产生的两个物候期。触角电位证实棉铃象甲触角对DMNT和TMTT的反应。行为测定，使用Y-管嗅觉仪，表明，添加合成的高萜烯生殖棉挥发物（模仿棉花在营养阶段的高萜烯水平）导致减少的吸引力棉铃象鼻虫相比，未改性的生殖棉。象鼻虫表现出没有偏好时，植物在营养阶段和营养阶段模仿植物之间的选择。结果表明，棉铃虫利用DMNT和TMTT来区分棉花的物候期。
Previous studies have shown that the boll weevil, Anthonomus grandis, is attracted to constitutive and conspecific herbivore-induced cotton volatiles, preferring the blend emitted by cotton at the reproductive over the vegetative stage. Moreover, this preference was paralleled by the release of the acyclic homoterpenes (tetranorterpenes) (E)-4,8-dimethyl-1,3,7-nonatriene (DMNT) and (E,E)-4,8,12-trimethyltrideca-1,3,7,11-tetraene (TMTT) in Delta Opal cotton being higher at the vegetative than at the reproductive stage. Here, we evaluated whether this difference in release of acyclic homoterpenes also occurred in other cotton varieties, and if boll weevils could recognize these compounds as indicators of a specific cotton phenological stage. Results showed that cotton genotypes CNPA TB-90, BRS-293 and Delta Opal all produced higher levels of DMNT and TMTT at the vegetative stage than at the reproductive stage and that these homoterpenes allowed for principal component analysis separation of volatiles produced by the two phenological stages. Electroantennograms confirmed boll weevil antennal responses to DMNT and TMTT. Behavioral assays, using Y-tube olfactometers, showed that adding synthetic homoterpenes to reproductive cotton volatiles (mimicking cotton at the vegetative stage in terms of homoterpene levels) resulted in reduced attraction to boll weevils compared to that to unmodified reproductive cotton. Weevils showed no preference when given a choice between plants at the vegetative stage and the vegetative stage-mimicked plant. Altogether, the results show that DMNT and TMTT are used by boll weevils to distinguish between cotton phenological stages.