THE RELATION OF RADIORESISTANCE TO BUDDING IN SACCHAROMYCES-CEREVISIAE
THE RELATION OF RADIORESISTANCE TO BUDDING IN SACCHAROMYCES-CEREVISIAE
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DOI：
10.1016/0003-9861(54)90172-1
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发表时间：
1954-01-01
影响因子：
3.9
通讯作者：
TOBIAS, CA
中科院分区：
文献类型：
--
作者：
BEAM, CA;MORTIMER, RK;TOBIAS, CA
单倍体酵母的x射线存活率由2个组成部分组成：初始指数（LD50 = 3100 r），代表分裂间细胞的存活率；耐辐射（LD50 = 60000 r）多次命中（靶数约1000）成分，代表分裂过程中细胞的存活率。细胞团块的能力，偶尔的二倍体细胞，以及辐射敏感性的分布来解释这种辐射抗性成分被消除。芽殖细胞代表抗辐射成分的证据有两个方面：1。在显微镜下观察单个细胞和出芽细胞的存活曲线，发现出芽细胞的抗辐射能力更强。2. 静止细胞悬浮液（饥饿2-5天，30[度]C，无可用氮）芽少，耐辐射成员少。当这些细胞被喂食时，新鲜芽的百分比与抗辐射成分的上升水平相关。
The X-ray survival of haploid yeast is shown to consist of 2 components: an initial exponential (LD50 = 3100 r) which represents the survival of interdivisional cells, and a radioresistant (LD50 = 60,000 r) multiple hit (target number ca. 1000) component which represents the survival of cells in the process of division. The ability of clumps of cells, occasional diploid cells, and a distribution of radiosensitivities to account for this radioresistant component are eliminated. Evidence that budding cells represent the radioresistant component is twofold: 1. Survival curves in which survival of single and budding cells from a growing culture is followed microscopically show budding cells to be more radioresistant. 2. Suspensions of resting cells (starved 2-5 d., 30[degree]C, without usable nitrogen) have few buds and few radioresistant members. When such cells are fed, the percentage of fresh buds is correlated with the rising level of the radioresistant component.